RATIONALE: Recently, a new asthma susceptibility gene, GPRA (G-protein-related receptor for asthma), has been identified by positional cloning. Initial association studies in a Finnish and Canadian population suggested an association with asthma and elevated serum IgE levels. OBJECTIVE: In a large, nested case-control study, associations between GPRA polymorphisms, asthma, and serum IgE levels were analyzed. METHODS: Using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technology, 1,872 German children aged 9 to 11 years (including 624 children with asthma and/or bronchial hyperresponsiveness) were genotyped for seven polymorphisms in the GPRA gene. MEASUREMENTS: Hardy-Weinberg equilibrium was assessed, and association studies with single nucleotide polymorphisms (SNPs) and haplotypes were performed. MAIN RESULTS: SNP 546333 increased the risk for asthma (odds ratio [OR], 1.40; 95% confidence interval [CI], 1.04-1.88; p = 0.025) and concomitant asthma and bronchial hyperresponsiveness (BHR; OR, 2.38; 95% CI, 1.22-4.66; p = 0.009). Also, SNP 585883 was associated with asthma (OR, 1.34; 95% CI, 1.04-1.72; p = 0.022) and asthma in combination with BHR (OR, 2.71; 95% CI, 1.45-5.09; p = 0.001). Furthermore, SNP 585883 was associated with elevated serum IgE levels (OR, 1.63; 95% CI, 1.10-2.42; p = 0.015). Haplotype combinations of risk alleles increased the OR for asthma to 1.83 (95% CI, 1.08-3.08; p = 0.024) and for asthma and concomitant BHR to OR 3.51 (95% CI, 1.08-11.37; p = 0.036). CONCLUSIONS: These results indicate that GPRA polymorphisms increase the susceptibility for asthma and BHR, and to a lesser degree for the elevation of serum IgE, in a German population, confirming initial observations in other white populations.
RATIONALE: Recently, a new asthma susceptibility gene, GPRA (G-protein-related receptor for asthma), has been identified by positional cloning. Initial association studies in a Finnish and Canadian population suggested an association with asthma and elevated serum IgE levels. OBJECTIVE: In a large, nested case-control study, associations between GPRA polymorphisms, asthma, and serum IgE levels were analyzed. METHODS: Using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technology, 1,872 German children aged 9 to 11 years (including 624 children with asthma and/or bronchial hyperresponsiveness) were genotyped for seven polymorphisms in the GPRA gene. MEASUREMENTS: Hardy-Weinberg equilibrium was assessed, and association studies with single nucleotide polymorphisms (SNPs) and haplotypes were performed. MAIN RESULTS: SNP 546333 increased the risk for asthma (odds ratio [OR], 1.40; 95% confidence interval [CI], 1.04-1.88; p = 0.025) and concomitant asthma and bronchial hyperresponsiveness (BHR; OR, 2.38; 95% CI, 1.22-4.66; p = 0.009). Also, SNP 585883 was associated with asthma (OR, 1.34; 95% CI, 1.04-1.72; p = 0.022) and asthma in combination with BHR (OR, 2.71; 95% CI, 1.45-5.09; p = 0.001). Furthermore, SNP 585883 was associated with elevated serum IgE levels (OR, 1.63; 95% CI, 1.10-2.42; p = 0.015). Haplotype combinations of risk alleles increased the OR for asthma to 1.83 (95% CI, 1.08-3.08; p = 0.024) and for asthma and concomitant BHR to OR 3.51 (95% CI, 1.08-11.37; p = 0.036). CONCLUSIONS: These results indicate that GPRA polymorphisms increase the susceptibility for asthma and BHR, and to a lesser degree for the elevation of serum IgE, in a German population, confirming initial observations in other white populations.
Authors: A S Amoah; B B Obeng; L May; Y C Kruize; I A Larbi; M Kabesch; M D Wilson; F C Hartgers; D A Boakye; M Yazdanbakhsh Journal: Genes Immun Date: 2014-05-22 Impact factor: 2.676
Authors: Martin Depner; Diana Hazard Taft; Pirkka V Kirjavainen; Karen M Kalanetra; Anne M Karvonen; Stefanie Peschel; Elisabeth Schmausser-Hechfellner; Caroline Roduit; Remo Frei; Roger Lauener; Amandine Divaret-Chauveau; Jean-Charles Dalphin; Josef Riedler; Marjut Roponen; Michael Kabesch; Harald Renz; Juha Pekkanen; Freda M Farquharson; Petra Louis; David A Mills; Erika von Mutius; Markus J Ege Journal: Nat Med Date: 2020-11-02 Impact factor: 53.440
Authors: H Wu; I Romieu; J-J Sienra-Monge; B E del Rio-Navarro; L Burdett; J Yuenger; H Li; S J Chanock; S J London Journal: Genes Immun Date: 2008-03-13 Impact factor: 2.676
Authors: Leonardo A Pinto; Martin Depner; Norman Klopp; Thomas Illig; Christian Vogelberg; Erika von Mutius; Michael Kabesch Journal: Respir Res Date: 2010-02-24
Authors: Sara L Richer; Ai Q Truong-Tran; David B Conley; Roderick Carter; David Vermylen; Leslie C Grammer; Anju T Peters; Rakesh K Chandra; Kathleen E Harris; Robert C Kern; Robert P Schleimer Journal: Am J Rhinol Date: 2008 May-Jun