BACKGROUND/AIMS: Hepatitis B virus (HBV), a major causative agent of hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that transcriptionally activates multiple viral and cellular promoters. How this regulation influences HCC is unclear. METHODS: Global gene expression in HBx-expressing and non-expressing hepatoma cells was analyzed using cDNA microarrays. RESULTS: Genes that were markedly up- or down-regulated in the presence of HBx included those involved in signal transduction, metabolism, apoptosis, cytokine production, cell cycle, cell adhesion and oncogenesis. Other genes of ill-defined function were also affected. Trans-activation proficient HBx up-regulated the transcription, translation and secretion of matrix metalloproteinase-3 (MMP-3), manifest as a cell migratory phenotype. This HBx effect was abrogated in the presence of a MMP-3 specific peptide inhibitor. CONCLUSIONS: HBx exerts selective transcriptional control in hepatoma cells and induces cellular migration through the activation of MMP-3.
BACKGROUND/AIMS: Hepatitis B virus (HBV), a major causative agent of hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that transcriptionally activates multiple viral and cellular promoters. How this regulation influences HCC is unclear. METHODS: Global gene expression in HBx-expressing and non-expressing hepatoma cells was analyzed using cDNA microarrays. RESULTS: Genes that were markedly up- or down-regulated in the presence of HBx included those involved in signal transduction, metabolism, apoptosis, cytokine production, cell cycle, cell adhesion and oncogenesis. Other genes of ill-defined function were also affected. Trans-activation proficient HBx up-regulated the transcription, translation and secretion of matrix metalloproteinase-3 (MMP-3), manifest as a cell migratory phenotype. This HBx effect was abrogated in the presence of a MMP-3 specific peptide inhibitor. CONCLUSIONS:HBx exerts selective transcriptional control in hepatoma cells and induces cellular migration through the activation of MMP-3.
Authors: Mark A Feitelson; Helena M G P V Reis; N Lale Tufan; Bill Sun; Jingbo Pan; Zhaorui Lian Journal: Cancer Lett Date: 2009-02-06 Impact factor: 8.679
Authors: Wing-Kin Sung; Yiwei Lu; Charlie W H Lee; Dongwei Zhang; Mostafa Ronaghi; Caroline G L Lee Journal: J Biol Chem Date: 2009-05-13 Impact factor: 5.157