Literature DB >> 1576236

Sensitive amplified immunoenzymometric assays (IEMA) for human insulin and intact proinsulin.

B Alpha1, L Cox, N Crowther, P M Clark, C N Hales.   

Abstract

Immunoenzymometric assays (IEMAs) for human insulin and intact proinsulin were developed using the amplification system developed by Johannsson et al. (Clin. Chim. Acta 148 (1985) 119-124) for the detection of the enzyme alkaline phosphatase. The detection limit of the assays was 0.8 pmol/l for proinsulin and 0.8 pmol/l for insulin whereas it was 1.8 pmol/l and 2.3 pmol/l respectively for the homologous immunoradiometric assays (IRMA). These assays are superior to immunoradiometric assays in terms of sensitivity, shelf-life of the labelled antibody and suitability for automation.

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Year:  1992        PMID： 1576236     DOI： 10.1515/cclm.1992.30.1.27

Source DB:  PubMed          Journal:  Eur J Clin Chem Clin Biochem        ISSN： 0939-4974


  11 in total

1.  Hypertriglyceridaemia in subjects with normal and abnormal glucose tolerance: relative contributions of insulin secretion, insulin resistance and suppression of plasma non-esterified fatty acids.

Authors:  C D Byrne; N J Wareham; D C Brown; P M Clark; L J Cox; N E Day; C R Palmer; T W Wang; D R Williams; C N Hales
Journal:  Diabetologia       Date:  1994-09       Impact factor: 10.122

2.  Fetal growth and impaired glucose tolerance in men and women.

Authors:  K Phipps; D J Barker; C N Hales; C H Fall; C Osmond; P M Clark
Journal:  Diabetologia       Date:  1993-03       Impact factor: 10.122

3.  Type 2 (non-insulin-dependent) diabetes mellitus, hypertension and hyperlipidaemia (syndrome X): relation to reduced fetal growth.

Authors:  D J Barker; C N Hales; C H Fall; C Osmond; K Phipps; P M Clark
Journal:  Diabetologia       Date:  1993-01       Impact factor: 10.122

4.  Decreased non-esterified fatty acid suppression and features of the insulin resistance syndrome occur in a sub-group of individuals with normal glucose tolerance.

Authors:  C D Byrne; N J Wareham; N E Day; R McLeish; D R Williams; C N Hales
Journal:  Diabetologia       Date:  1995-11       Impact factor: 10.122

5.  Fetal growth and insulin secretion in adult life.

Authors:  D I Phillips; S Hirst; P M Clark; C N Hales; C Osmond
Journal:  Diabetologia       Date:  1994-06       Impact factor: 10.122

Review 6.  The pathogenesis of NIDDM.

Authors:  C N Hales
Journal:  Diabetologia       Date:  1994-09       Impact factor: 10.122

7.  Adiposity, inflammation and hyperglycaemia in rural and urban Indian men: Coronary Risk of Insulin Sensitivity in Indian Subjects (CRISIS) Study.

Authors:  C S Yajnik; C V Joglekar; H G Lubree; S S Rege; S S Naik; D S Bhat; B Uradey; K N Raut; P Shetty; J S Yudkin
Journal:  Diabetologia       Date:  2007-10-31       Impact factor: 10.122

8.  Associations of trunk fat depots with insulin resistance, β cell function and glycaemia--a multiple technique study.

Authors:  Anjali Ganpule-Rao; Charudatta Joglekar; Deepak Patkar; Manoj Chinchwadkar; Dattatreya Bhat; Himangi Lubree; Sonali Rege; Bhagyashree Uradey; Chittaranjan Yajnik; John Yudkin
Journal:  PLoS One       Date:  2013-10-08       Impact factor: 3.240

9.  Vitamin D insufficiency is common in Indian mothers but is not associated with gestational diabetes or variation in newborn size.

Authors:  H J W Farrant; G V Krishnaveni; J C Hill; B J Boucher; D J Fisher; K Noonan; C Osmond; S R Veena; C H D Fall
Journal:  Eur J Clin Nutr       Date:  2008-02-20       Impact factor: 4.016

10.  Candidate gene association study in type 2 diabetes indicates a role for genes involved in beta-cell function as well as insulin action.

Authors:  Inês Barroso; Jian'an Luan; Rita P S Middelberg; Anne-Helen Harding; Paul W Franks; Rupert W Jakes; D Clayton; Alan J Schafer; Stephen O'Rahilly; Nicholas J Wareham
Journal:  PLoS Biol       Date:  2003-10-13       Impact factor: 8.029
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