| Literature DB >> 15761609 |
Regina M S Amorim1, Claudiner P Oliveira, Patrícia S Wyant, Daniela M Cerqueira, Geni N L Câmara, Luciano S Flores, Regina M B Martins, Cláudia R F Martins.
Abstract
The objective of this study was to characterize hepatitis C virus (HCV) genotypes in blood donors from the Federal District, Central Brazil, and to compare HCV screening by serological assays and reverse transcriptase polymerase chain reaction (RT-PCR). Plasma samples from 57 individuals with reactive or indeterminate results in serological anti-HCV screening assays (ELISA or EIA) were tested for HCV RNA by RT-PCR. The results from a confirmatory LIA serological assay were also evaluated. The 5' non-coding region of the HCV genome was amplified from 41 PCR positive samples (71.9%), which were further characterized by nucleotide sequencing analysis. Of these, 60.9% were of HCV genotype 1 and 39.1% of genotype 3.Entities:
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Year: 2005 PMID： 15761609 DOI： 10.1590/s0074-02762004000800019
Source DB: PubMed Journal: Mem Inst Oswaldo Cruz ISSN： 0074-0276 Impact factor: 2.743