Literature DB >> 15750129

Comparison of COBAS AMPLICOR Neisseria gonorrhoeae PCR, including confirmation with N. gonorrhoeae-specific 16S rRNA PCR, with traditional culture.

Dirk S Luijt1, Petra A J Bos, Anton A van Zwet, Pieter C van Voorst Vader, Jurjen Schirm.   

Abstract

A total of 3,023 clinical specimens were tested for Neisseria gonorrhoeae by using COBAS AMPLICOR (CA) PCR and confirmation of positives by N. gonorrhoeae-specific 16S rRNA PCR. The sensitivity of CA plus 16S rRNA PCR was 98.8%, compared to 68.2% for culture. Confirmation of CA positives increased the positive predictive value from 54.8 to 96.6%.

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Year:  2005        PMID: 15750129      PMCID: PMC1081235          DOI: 10.1128/JCM.43.3.1445-1447.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN: 0095-1137            Impact factor:   5.948


  11 in total

1.  Confirmation by 16S rRNA PCR of the COBAS AMPLICOR CT/NG test for diagnosis of Neisseria gonorrhoeae infection in a low-prevalence population.

Authors:  David J Diemert; Michael D Libman; Pierre Lebel
Journal:  J Clin Microbiol       Date:  2002-11       Impact factor: 5.948

2.  Multicenter evaluation of the BDProbeTec ET System for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine specimens, female endocervical swabs, and male urethral swabs.

Authors:  B Van Der Pol; D V Ferrero; L Buck-Barrington; E Hook; C Lenderman; T Quinn; C A Gaydos; J Lovchik; J Schachter; J Moncada; G Hall; M J Tuohy; R B Jones
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

3.  Comparison between the LCx Probe system and the COBAS AMPLICOR system for detection of Chlamydia trachomatis and Neisseria gonorrhoeae infections in patients attending a clinic for treatment of sexually transmitted diseases in Amsterdam, The Netherlands.

Authors:  G J van Doornum; L M Schouls; A Pijl; I Cairo; M Buimer; S Bruisten
Journal:  J Clin Microbiol       Date:  2001-03       Impact factor: 5.948

4.  Detection of Neisseria gonorrhoeae and Chlamydia trachomatis in genitourinary specimens from men and women by a coamplification PCR assay.

Authors:  K A Crotchfelt; L E Welsh; D DeBonville; M Rosenstraus; T C Quinn
Journal:  J Clin Microbiol       Date:  1997-06       Impact factor: 5.948

5.  Enhancing the specificity of the COBAS AMPLICOR CT/NG test for Neisseria gonorrhoeae by retesting specimens with equivocal results.

Authors:  B Van Der Pol; D H Martin; J Schachter; T C Quinn; C A Gaydos; R B Jones; K Crotchfelt; J Moncada; D Jungkind; B Turner; C Peyton; J F Kelly; J B Weiss; M Rosenstraus
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

6.  Multicenter evaluation of AMPLICOR and automated COBAS AMPLICOR CT/NG tests for Neisseria gonorrhoeae.

Authors:  D H Martin; C Cammarata; B Van Der Pol; R B Jones; T C Quinn; C A Gaydos; K Crotchfelt; J Schachter; J Moncada; D Jungkind; B Turner; C Peyton
Journal:  J Clin Microbiol       Date:  2000-10       Impact factor: 5.948

7.  Evaluation of the Abbott LCx ligase chain reaction assay for detection of Chlamydia trachomatis and Neisseria gonorrhoeae in urine and genital swab specimens from a sexually transmitted disease clinic population.

Authors:  K C Carroll; W E Aldeen; M Morrison; R Anderson; D Lee; S Mottice
Journal:  J Clin Microbiol       Date:  1998-06       Impact factor: 5.948

8.  The effect of urine testing in evaluations of the sensitivity of the Gen-Probe Aptima Combo 2 assay on endocervical swabs for Chlamydia trachomatis and neisseria gonorrhoeae: the infected patient standard reduces sensitivity of single site evaluation.

Authors:  Jeanne Moncada; Julius Schachter; Edward W Hook; Dennis Ferrero; Charlotte Gaydos; Thomas C Quinn; Dean Willis; Alice Weissfeld; David H Martin
Journal:  Sex Transm Dis       Date:  2004-05       Impact factor: 2.830

9.  Evaluation of AMPLICOR Neisseria gonorrhoeae PCR using cppB nested PCR and 16S rRNA PCR.

Authors:  D J Farrell
Journal:  J Clin Microbiol       Date:  1999-02       Impact factor: 5.948

10.  Multicenter evaluation of the fully automated COBAS AMPLICOR PCR test for detection of Chlamydia trachomatis in urogenital specimens.

Authors:  J Vincelette; J Schirm; M Bogard; A M Bourgault; D S Luijt; A Bianchi; P C van Voorst Vader; A Butcher; M Rosenstraus
Journal:  J Clin Microbiol       Date:  1999-01       Impact factor: 5.948

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  7 in total

1.  Loop-mediated isothermal amplification test for detection of Neisseria gonorrhoeae in urine samples and tolerance of the assay to the presence of urea.

Authors:  Thomas Edwards; Patricia A Burke; Helen B Smalley; Liz Gillies; Glyn Hobbs
Journal:  J Clin Microbiol       Date:  2014-03-12       Impact factor: 5.948

2.  Evaluation of a rapid point-of-care test for the detection of gonococcal infection among female sex workers in Benin.

Authors:  M Alary; C Gbenafa-Agossa; G Aïna; M Ndour; A C Labbé; D Fortin; M Steele; R W Peeling
Journal:  Sex Transm Infect       Date:  2006-12       Impact factor: 3.519

3.  A fast real-time polymerase chain reaction method for sensitive and specific detection of the Neisseria gonorrhoeae porA pseudogene.

Authors:  Stig Ove Hjelmevoll; Merethe Elise Olsen; Johanna U Ericson Sollid; Håkon Haaheim; Magnus Unemo; Vegard Skogen
Journal:  J Mol Diagn       Date:  2006-11       Impact factor: 5.568

4.  Aptima Combo 2 testing detected additional cases of Neisseria gonorrhoeae infection in men and women in community settings.

Authors:  Julius Kapala; Kathy Biers; Michael Cox; Margaret Kamionka; Jeff Sumner; Ramandeep Toor; Jodi Gilchrist; Dan Jang; Marek Smieja; Max Chernesky
Journal:  J Clin Microbiol       Date:  2011-03-16       Impact factor: 5.948

5.  Neisseria species identification assay for the confirmation of Neisseria gonorrhoeae-positive results of the COBAS Amplicor PCR.

Authors:  Kathy A Mangold; MaryAnn Regner; Mohammed Tajuddin; Aamair M Tajuddin; Lawrence Jennings; Hongyan Du; Karen L Kaul
Journal:  J Clin Microbiol       Date:  2007-03-14       Impact factor: 5.948

6.  Use of the mtrR Gene for Rapid Molecular Diagnosis of Neisseria gonorrhoeae and Identification of the Reduction of Susceptibility to Antibiotics in Endocervical Swabs.

Authors:  Marcos R Escobedo-Guerra; Mitzuko Katoku-Herrera; Marcela Lopez-Hurtado; Rodrigo Gutierrez-Trujillo; Fernando M Guerra-Infante
Journal:  Mol Diagn Ther       Date:  2018-06       Impact factor: 4.074

7.  Loop-mediated isothermal amplification of Neisseria gonorrhoeae porA pseudogene: a rapid and reliable method to detect gonorrhea.

Authors:  Mei-Ling Liu; Yong Xia; Xing-Zhong Wu; Jian-Qiong Huang; Xu-Guang Guo
Journal:  AMB Express       Date:  2017-02-23       Impact factor: 3.298

  7 in total

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