Literature DB >> 15749051

Identification, cloning, and functional expression of three glutathione transferase genes from Aspergillus fumigatus.

Claire Burns1, Rachel Geraghty, Claire Neville, Alan Murphy, Kevin Kavanagh, Sean Doyle.   

Abstract

Analysis of the genome of the human pathogen, Aspergillus fumigatus, revealed the presence of several putative glutathione transferase (GST) open reading frames. Three A. fumigatus GST genes, termed gstA, B, and C, were cloned and recombinant proteins expressed in Escherichia coli. Functional analysis of recombinant gstA-C confirms that the enzymes exhibit GST activity and glutathione peroxidase activity. RT-PCR confirmed low basal expression of gstA and gstC which was markedly up-regulated (at least 4x-10x) in the presence of either H2O2 or 1-chloro-2,4-dinitrobenzene (CDNB). GstB expression was only observed in the presence of CDNB. These results demonstrate for the first time the existence of three functional GSTs in A. fumigatus and strongly suggest a role for these enzymes in the response of the organism to both oxidative stress and xenobiotic presence.

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Year:  2005        PMID： 15749051     DOI： 10.1016/j.fgb.2005.01.001

Source DB:  PubMed          Journal:  Fungal Genet Biol        ISSN： 1087-1845            Impact factor:   3.495


  26 in total

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Authors:  Márcia Eliana da Silva Ferreira; Iran Malavazi; Marcela Savoldi; Axel A Brakhage; Maria Helena S Goldman; H Stanley Kim; William C Nierman; Gustavo H Goldman
Journal:  Curr Genet       Date:  2006-04-19       Impact factor: 3.886

2.  Nonribosomal peptide synthetase genes pesL and pes1 are essential for Fumigaclavine C production in Aspergillus fumigatus.

Authors:  Karen A O'Hanlon; Lorna Gallagher; Markus Schrettl; Christoph Jöchl; Kevin Kavanagh; Thomas O Larsen; Sean Doyle
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3.  Targeted disruption of nonribosomal peptide synthetase pes3 augments the virulence of Aspergillus fumigatus.

Authors:  Karen A O'Hanlon; Timothy Cairns; Deirdre Stack; Markus Schrettl; Elaine M Bignell; Kevin Kavanagh; Sinéad M Miggin; Grainne O'Keeffe; Thomas O Larsen; Sean Doyle
Journal:  Infect Immun       Date:  2011-07-11       Impact factor: 3.441

4.  Exploration of Sulfur Assimilation of Aspergillus fumigatus Reveals Biosynthesis of Sulfur-Containing Amino Acids as a Virulence Determinant.

Authors:  Jorge Amich; Michaela Dümig; Gráinne O'Keeffe; Jasmin Binder; Sean Doyle; Andreas Beilhack; Sven Krappmann
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6.  The glutathione system of Aspergillus nidulans involves a fungus-specific glutathione S-transferase.

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Journal:  J Biol Chem       Date:  2009-01-26       Impact factor: 5.157

7.  Expression of HPV-16 L1 capsomeres with glutathione-S-transferase as a fusion protein in tobacco plastids: an approach for a capsomere-based HPV vaccine.

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Journal:  Hum Vaccin Immunother       Date:  2014-11-19       Impact factor: 3.452

8.  The Aspergillus fumigatus protein GliK protects against oxidative stress and is essential for gliotoxin biosynthesis.

Authors:  Lorna Gallagher; Rebecca A Owens; Stephen K Dolan; Grainne O'Keeffe; Markus Schrettl; Kevin Kavanagh; Gary W Jones; Sean Doyle
Journal:  Eukaryot Cell       Date:  2012-08-17

9.  Comparison of glutathione S-transferase activity and concentration in aflatoxin-producing and their non-toxigenic counterpart isolates.

Authors:  Tahereh Ziglari; Abdolamir Allameh; Mehdi Razzaghi-Abyaneh; Ali-Reza Khosravi; Mohammad-Hossein Yadegari
Journal:  Mycopathologia       Date:  2008-07-12       Impact factor: 2.574

10.  Characterization of a Phanerochaete chrysosporium glutathione transferase reveals a novel structural and functional class with ligandin properties.

Authors:  Yann Mathieu; Pascalita Prosper; Marc Buée; Stéphane Dumarçay; Frédérique Favier; Eric Gelhaye; Philippe Gérardin; Luc Harvengt; Jean-Pierre Jacquot; Tiphaine Lamant; Edgar Meux; Sandrine Mathiot; Claude Didierjean; Mélanie Morel
Journal:  J Biol Chem       Date:  2012-09-24       Impact factor: 5.157
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