Literature DB >> 15748766

Immobilization of Candida rugosa lipase on a pH-sensitive support for enantioselective hydrolysis of ketoprofen ester.

Shengdong Zhu1, Yuanxin Wu, Ziniu Yu.   

Abstract

Candida rugosa lipase (Lipase OF) was immobilized by covalent binding to a pH-sensitive support showing reversibly soluble-insoluble characteristics with pH change. The immobilized lipase could carry out the enantioselective hydrolysis of ketoprofen ester in a soluble form yet be recovered after precipitation by simply adjusting pH. Its activity and enantioselectivity for hydrolysis of 2-chloroethyl ester of ketoprofen were enhanced 1.5-fold and 8.7-fold compared with those of free lipase. After eight catalytic cycles, the immobilized enzyme was still 46% active and its enantioselectivity remained unchanged.

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Year:  2005        PMID： 15748766     DOI： 10.1016/j.jbiotec.2004.12.012

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  4 in total

1.  Characterization, crystallization and preliminary X-ray diffraction analysis of an (S)-specific esterase (pfEstA) from Pseudomonas fluorescens KCTC 1767: enantioselectivity for potential industrial applications.

Authors:  Seulgi Kim; Tri Duc Ngo; Kyeong Kyu Kim; T Doohun Kim
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2012-10-30

2.  Purification, crystallization and preliminary crystallographic analysis of Est25: a ketoprofen-specific hormone-sensitive lipase.

Authors:  SeungBum Kim; Sangbum Joo; Hyun C Yoon; Yeonwoo Ryu; Kyeong Kyu Kim; T Doohun Kim
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-06-11

3.  Immobilization of Yarrowia lipolytica Lipase on Macroporous Resin Using Different Methods: Characterization of the Biocatalysts in Hydrolysis Reaction.

Authors:  Jingjing Sun; Yiling Chen; Jun Sheng; Mi Sun
Journal:  Biomed Res Int       Date:  2015-07-09       Impact factor: 3.411

4.  Catalyzed ester synthesis using Candida rugosa lipase entrapped by poly(N-isopropylacrylamide-co-itaconic acid) hydrogel.

Authors:  Nikola Milašinović; Sonja Jakovetić; Zorica Knežević-Jugović; Nedeljko Milosavljević; Marija Lučić; Jovanka Filipović; Melina Kalagasidis Krušić
Journal:  ScientificWorldJournal       Date:  2014-02-20
  4 in total

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