The identification and characterization of a break within the large subunit ribosomal RNA of Trichinella spiralis: comparison of gap sequences within the genus.

A break was identified in the large subunit ribosomal RNA of Trichinella spiralis that results in its dissociation into 2 smaller fragments of approximately equal length. The approximate location of the break within the encoding gene was mapped from subcloned rDNA fragments by S1 protection experiments. The boundaries of the break were determined by cDNA primer extension and S1 nuclease protection assays. The excised fragment (gap sequence) was localized to expansion segment 5 within domain IV from which 86 bases are removed during the excision process. The gap region is flanked by the consensus sequence CGAAAG; however, comparison of expansion segment 5 sequences from T. spiralis, T. nativa, T. nelsoni and T. pseudospiralis, all of which undergo 'gap processing', demonstrates significant size and sequence heterogeneity and provides little evidence for additional consensus sequences which could be implicated in gap processing.