Literature DB >> 15740853

Comparison of a commercial ELISA with the modified agglutination test for detection of Toxoplasma infection in the domestic pig.

H R Gamble1, J P Dubey, D N Lambillotte.   

Abstract

The modified agglutination test (MAT) and a commercially available enzyme-linked immunosorbent assay (ELISA) were compared for detection of antibodies to Toxoplasma gondii in naturally-infected market-aged pigs. Infected pigs were obtained from commercial slaughter facilities and from farms where infection had previously been detected. Infection was confirmed by bioassay in cats. For 70 bioassay positive pigs, 60 were positive by MAT (85.7% sensitivity) and 62 were positive by ELISA (88.6% sensitivity). Of 204 bioassay negative samples 193 were negative by MAT (94.6% specificity) and 200 were negative by ELISA (98.0% specificity). Good correlation was seen between MAT and ELISA results. The results suggest that the ELISA may be a good tool for epidemiological studies of Toxoplasma infection on pig farms.

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Year:  2005        PMID: 15740853     DOI: 10.1016/j.vetpar.2004.11.019

Source DB:  PubMed          Journal:  Vet Parasitol        ISSN: 0304-4017            Impact factor:   2.738


  28 in total

1.  Detection of Toxoplasma gondii in small ruminants in Chennai using PCR and modified direct agglutination test.

Authors:  Ajay Suryakant Satbige; M Vijaya Bharathi; P I Ganesan; C Sreekumar; C Rajendran
Journal:  J Parasit Dis       Date:  2016-01-13

2.  Detection of Toxoplasma gondii in naturally infected domestic pigs in Northern Serbia.

Authors:  Ljiljana Kuruca; Ivana Klun; Aleksandra Uzelac; Aleksandra Nikolić; Branko Bobić; Stanislav Simin; Vesna Lalošević; Dušan Lalošević; Olgica Djurković-Djaković
Journal:  Parasitol Res       Date:  2017-09-27       Impact factor: 2.289

3.  Seroprevalence of Toxoplasma gondii in Iberian pig sows.

Authors:  Alba Pablos-Tanarro; Luis Miguel Ortega-Mora; Antonio Palomo; Francisco Casasola; Ignacio Ferre
Journal:  Parasitol Res       Date:  2018-03-13       Impact factor: 2.289

4.  Identification of a sporozoite-specific antigen from Toxoplasma gondii.

Authors:  Dolores Hill; Cathleen Coss; J P Dubey; Kristen Wroblewski; Mari Sautter; Tiffany Hosten; Claudia Muñoz-Zanzi; Ernest Mui; Shawn Withers; Kenneth Boyer; Gretchen Hermes; Jessica Coyne; Frank Jagdis; Andrew Burnett; Patrick McLeod; Holmes Morton; Donna Robinson; Rima McLeod
Journal:  J Parasitol       Date:  2011-03-03       Impact factor: 1.276

5.  Pig and herd level prevalence of Toxoplasma gondii in Ontario finisher pigs in 2001, 2003, and 2004.

Authors:  Zvonimir Poljak; Catherine E Dewey; Robert M Friendship; S Wayne Martin; Jette Christensen; Davor Ojkic; John Wu; Eva Chow
Journal:  Can J Vet Res       Date:  2008-07       Impact factor: 1.310

6.  Significance of a common 65 kDa antigen in the experimental fasciolosis and toxoplasmosis.

Authors:  Raafat Mohamed Shaapan; Nagwa Ibrahim Toaleb; Eman Hussein Abdel-Rahman
Journal:  J Parasit Dis       Date:  2013-11-20

Review 7.  Global seroprevalence of Toxoplasma gondii in Camelidae: A systematic review and meta-analysis.

Authors:  Nahid Maspi; Tooran Nayeri; Mahmood Moosazadeh; Shahabeddin Sarvi; Mehdi Sharif; Ahmad Daryani
Journal:  Acta Parasitol       Date:  2021-03-05       Impact factor: 1.440

Review 8.  Descriptive Comparison of ELISAs for the Detection of Toxoplasma gondii Antibodies in Animals: A Systematic Review.

Authors:  K L D Tharaka D Liyanage; Anke Wiethoelter; Jasmin Hufschmid; Abdul Jabbar
Journal:  Pathogens       Date:  2021-05-15

9.  Comparison of a Commercial ELISA with the Modified Agglutination Test for Detection of Toxoplasma gondii Antibodies in Sera of Naturally Infected Dogs and Cats.

Authors:  Ch Zhu; Ll Cui; Ls Zhang
Journal:  Iran J Parasitol       Date:  2012       Impact factor: 1.012

10.  Evaluation of a commercial ELISA kit for detection of antibodies against Toxoplasma gondii in serum, plasma and meat juice from experimentally and naturally infected sheep.

Authors:  Sabine B Glor; Renate Edelhofer; Felix Grimm; Peter Deplazes; Walter Basso
Journal:  Parasit Vectors       Date:  2013-04-05       Impact factor: 3.876

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