BACKGROUND: Altered serotonergic (5-HT) neurotransmission is implicated in the antidepressant and anxiolytic properties of physical activity. In the current study, we investigated whether physical activity alters factors involved in the regulation of central 5-HT neural activity. METHODS: In situ hybridization was used to quantify levels of 5-HT transporter (5-HTT), 5-HT(1A), 5-HT(1B), and alpha(1b)-adrenergic receptor (alpha(1b) ADR) messenger ribonucleic acids (mRNAs) in the dorsal (DRN) and median raphe (MR) nuclei of male Fischer rats after either sedentary housing or 3 days, 3 weeks, or 6 weeks of wheel running. RESULTS: Wheel running produced a rapid and lasting reduction of 5-HT(1B) mRNA in the ventral DRN. Three weeks of wheel running decreased 5-HTT mRNA in the DRN and MR and increased alpha(1b) ADR mRNA in the DRN. After 6 weeks of wheel running, 5-HTT mRNA remained reduced, but alpha(1b) ADR mRNA returned to sedentary levels. Serotonin(1A) mRNA was increased in the MR and certain DRN subregions after 6 weeks only. CONCLUSIONS: Data suggest that the central 5-HT system is sensitive to wheel running in a time-dependent manner. The observed changes in mRNA regulation in a subset of raphe nuclei might contribute to the stress resistance produced by wheel running and the antidepressant and anxiolytic effects of physical activity.
BACKGROUND: Altered serotonergic (5-HT) neurotransmission is implicated in the antidepressant and anxiolytic properties of physical activity. In the current study, we investigated whether physical activity alters factors involved in the regulation of central 5-HT neural activity. METHODS: In situ hybridization was used to quantify levels of 5-HT transporter (5-HTT), 5-HT(1A), 5-HT(1B), and alpha(1b)-adrenergic receptor (alpha(1b) ADR) messenger ribonucleic acids (mRNAs) in the dorsal (DRN) and median raphe (MR) nuclei of male Fischer rats after either sedentary housing or 3 days, 3 weeks, or 6 weeks of wheel running. RESULTS: Wheel running produced a rapid and lasting reduction of 5-HT(1B) mRNA in the ventral DRN. Three weeks of wheel running decreased 5-HTT mRNA in the DRN and MR and increased alpha(1b) ADR mRNA in the DRN. After 6 weeks of wheel running, 5-HTT mRNA remained reduced, but alpha(1b) ADR mRNA returned to sedentary levels. Serotonin(1A) mRNA was increased in the MR and certain DRN subregions after 6 weeks only. CONCLUSIONS: Data suggest that the central 5-HT system is sensitive to wheel running in a time-dependent manner. The observed changes in mRNA regulation in a subset of raphe nuclei might contribute to the stress resistance produced by wheel running and the antidepressant and anxiolytic effects of physical activity.
Authors: Benjamin N Greenwood; Teresa E Foley; Tony V Le; Paul V Strong; Alice B Loughridge; Heidi E W Day; Monika Fleshner Journal: Behav Brain Res Date: 2010-11-09 Impact factor: 3.332
Authors: R Parrish Waters; R B Pringle; G L Forster; K J Renner; J L Malisch; T Garland; J G Swallow Journal: Brain Res Date: 2013-01-23 Impact factor: 3.252
Authors: Stefan O Reber; Philip H Siebler; Nina C Donner; James T Morton; David G Smith; Jared M Kopelman; Kenneth R Lowe; Kristen J Wheeler; James H Fox; James E Hassell; Benjamin N Greenwood; Charline Jansch; Anja Lechner; Dominic Schmidt; Nicole Uschold-Schmidt; Andrea M Füchsl; Dominik Langgartner; Frederick R Walker; Matthew W Hale; Gerardo Lopez Perez; Will Van Treuren; Antonio González; Andrea L Halweg-Edwards; Monika Fleshner; Charles L Raison; Graham A Rook; Shyamal D Peddada; Rob Knight; Christopher A Lowry Journal: Proc Natl Acad Sci U S A Date: 2016-05-16 Impact factor: 11.205