Literature DB >> 1572935

A rapid method for purification of herpesvirus DNA.

L S Christensen1, P Normann.   

Abstract

A rapid and reliable method for purification of herpesvirus DNA from cell cultures is described. The method is based on the isolation of virus particles and/or nucleocapsids by differential centrifugation and exploits the solubilizing and denaturing capabilities of cesium trifluoroacetate during isopycnic centrifugation, so that phenol/chloroform extractions can be omitted. The method was used for the purification of DNA from several members of the Alfaherpesvirinae subfamily.

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Year:  1992        PMID： 1572935     DOI： 10.1016/0166-0934(92)90024-8

Source DB:  PubMed          Journal:  J Virol Methods        ISSN： 0166-0934            Impact factor:   2.014


  4 in total

1.  Bovine herpesvirus type 2 is closely related to the primate alphaherpesviruses.

Authors:  B Ehlers; M Goltz; M P Ejercito; G K Dasika; G J Letchworth
Journal:  Virus Genes       Date:  1999       Impact factor: 2.332

2.  Establishment of conditions for the detection of bovine herpesvirus-1 by polymerase chain reaction using primers in the thymidine kinase region.

Authors:  C V Yason; L M Harris; P K McKenna; D Wadowska; F S Kibenge
Journal:  Can J Vet Res       Date:  1995-04       Impact factor: 1.310

3.  High frequency intergenomic recombination of suid herpesvirus 1 (SHV-1, Aujeszky's disease virus).

Authors:  L S Christensen; B Lomniczi
Journal:  Arch Virol       Date:  1993       Impact factor: 2.574

4.  Reintroduction of bovine herpes virus type 1 into Danish cattle herds during the period 1991-1995: a review of the investigations in the infected herds.

Authors:  B Nylin; K G Madsen; L Rønsholt
Journal:  Acta Vet Scand       Date:  1998       Impact factor: 1.695
  4 in total

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