OBJECTIVE: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. SUMMARY BACKGROUND DATA: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. METHODS: Canine BMCs were differentiated in vitro into smooth muscle alpha-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donor dogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. RESULTS: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. CONCLUSIONS: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.
OBJECTIVE: The objective of this study is to investigate if bone marrow-derived cells (BMCs) regenerate vascular tissues and improve patency in tissue-engineered small-diameter (internal diameter = 3 mm) vascular grafts. SUMMARY BACKGROUND DATA: BMCs have demonstrated the ability to differentiate into endothelial-like cells and vascular smooth muscle-like cells and may offer an alternative cell source for vascular tissue engineering. Thus, we tissue-engineered small-diameter vascular grafts with BMCs and decellularized arteries. METHODS:CanineBMCs were differentiated in vitro into smooth muscle alpha-actin/smooth muscle myosin heavy-chain-positive cells and von Willebrand factor/CD31-positive cells and seeded onto decellularized canine carotid arteries (internal diameter = 3 mm). The seeded grafts were implanted in cell donordogs. The vascular-tissue regeneration and graft patency were investigated with immunohistochemistry and angiography, respectively. RESULTS: The vascular grafts seeded with BMCs remained patent for up to 8 weeks in the canine carotid artery interposition model, whereas nonseeded grafts occluded within 2 weeks. Within 8 weeks after implantation, the vascular grafts showed regeneration of the 3 elements of artery (endothelium, media, and adventitia). BMCs labeled with a fluorescent dye prior to implantation were detected in the retrieved vascular grafts, indicating that the BMCs participated in the vascular tissue regeneration. CONCLUSIONS: Here we show that BMCs have the potential to regenerate vascular tissues and improve patency in tissue-engineered small-diameter vascular grafts. This is the first report of a small-diameter neovessel engineered with BMCs as a cell source.
Authors: A Bader; G Steinhoff; K Strobl; T Schilling; G Brandes; H Mertsching; D Tsikas; J Froelich; A Haverich Journal: Transplantation Date: 2000-07-15 Impact factor: 4.939
Authors: John A Hawkins; Neal D Hillman; Linda M Lambert; Jamison Jones; Gregory B Di Russo; Tracie Profaizer; Thomas C Fuller; L LuAnn Minich; Richard V Williams; Robert E Shaddy Journal: J Thorac Cardiovasc Surg Date: 2003-07 Impact factor: 5.209
Authors: Christof Stamm; Bernd Westphal; Hans-Dieter Kleine; Michael Petzsch; Christian Kittner; Heiko Klinge; Carl Schümichen; Christoph A Nienaber; Mathias Freund; Gustav Steinhoff Journal: Lancet Date: 2003-01-04 Impact factor: 79.321
Authors: Sandip Sarkar; Thomas Schmitz-Rixen; George Hamilton; Alexander M Seifalian Journal: Med Biol Eng Comput Date: 2007-03-06 Impact factor: 2.602
Authors: Gustavo A Villalona; Brooks Udelsman; Daniel R Duncan; Edward McGillicuddy; Rajendra F Sawh-Martinez; Narutoshi Hibino; Christopher Painter; Tamar Mirensky; Benjamin Erickson; Toshiharu Shinoka; Christopher K Breuer Journal: Tissue Eng Part B Rev Date: 2010-06 Impact factor: 6.389