PURPOSE: To characterize a novel gene, deleted in polyposis 1-like 1 (Dp1l1), which is expressed in the retina. METHODS: A systematic screening by subtraction hybridization of the cDNAs from mouse retina and mouse brain was performed to obtain novel genes expressed in the retina. In situ hybridization, immunohistochemistry, and intracellular localization analyses were performed to investigate the expression patterns of Dp1l1. The chromosomal location of Dp1l1 was determined by radiation hybrid mapping. Bioinformatics was used for homology analysis. RESULTS: A novel gene, Dp1l1, was expressed abundantly in the retina. It encodes a 201-amino-acid protein, and the encoded protein is designated mouse TB2-like 1. It is highly homologous to the mouse TB2, which is encoded by deleted in polyposis 1 (Dp1). In situ hybridization and immunohistochemical analyses showed that Dp1l1 mRNA and the TB2-like 1 were localized richly in retinal ganglion cells (RGCs). TB2-like 1 was present in the cytoplasm in a punctate pattern. Dp1l1 was mapped to mouse chromosome 10 by radiation hybrid mapping. CONCLUSIONS: TB2-like 1 is a membrane protein that belongs to the YOP1/TB2/DP1/HVA22 family, and it probably plays an important role in intracellular membrane trafficking in RGCs, based on the properties of other homologous proteins.
PURPOSE: To characterize a novel gene, deleted in polyposis 1-like 1 (Dp1l1), which is expressed in the retina. METHODS: A systematic screening by subtraction hybridization of the cDNAs from mouse retina and mouse brain was performed to obtain novel genes expressed in the retina. In situ hybridization, immunohistochemistry, and intracellular localization analyses were performed to investigate the expression patterns of Dp1l1. The chromosomal location of Dp1l1 was determined by radiation hybrid mapping. Bioinformatics was used for homology analysis. RESULTS: A novel gene, Dp1l1, was expressed abundantly in the retina. It encodes a 201-amino-acid protein, and the encoded protein is designated mouse TB2-like 1. It is highly homologous to the mouse TB2, which is encoded by deleted in polyposis 1 (Dp1). In situ hybridization and immunohistochemical analyses showed that Dp1l1 mRNA and the TB2-like 1 were localized richly in retinal ganglion cells (RGCs). TB2-like 1 was present in the cytoplasm in a punctate pattern. Dp1l1 was mapped to mouse chromosome 10 by radiation hybrid mapping. CONCLUSIONS: TB2-like 1 is a membrane protein that belongs to the YOP1/TB2/DP1/HVA22 family, and it probably plays an important role in intracellular membrane trafficking in RGCs, based on the properties of other homologous proteins.
Authors: Y Ann Chen; Jonas S Almeida; Adam J Richards; Peter Müller; Raymond J Carroll; Baerbel Rohrer Journal: J Comput Graph Stat Date: 2010-09-01 Impact factor: 2.302
Authors: Hong Hao; Shobi Veleri; Bo Sun; Douglas S Kim; Patrick W Keeley; Jung-Woong Kim; Hyun-Jin Yang; Sharda P Yadav; Souparnika H Manjunath; Raman Sood; Paul Liu; Benjamin E Reese; Anand Swaroop Journal: Hum Mol Genet Date: 2014-04-01 Impact factor: 6.150
Authors: Smriti A Agrawal; Thomas Burgoyne; Aiden Eblimit; James Bellingham; David A Parfitt; Amelia Lane; Ralph Nichols; Chinwe Asomugha; Matthew J Hayes; Peter M Munro; Mingchu Xu; Keqing Wang; Clare E Futter; Yumei Li; Rui Chen; Michael E Cheetham Journal: Hum Mol Genet Date: 2017-07-15 Impact factor: 6.150