F Rijken1, R C M Kiekens, P L B Bruijnzeel. 1. Department of Dermatology, University Medical Centre Utrecht, Heidelberglaan 100, 3584 CX Utrecht, the Netherlands.
Abstract
BACKGROUND: The pathophysiology of photoageing of the skin has been studied extensively. Matrix metalloproteinases (MMPs) originating from keratinocytes and fibroblasts are thought to play a primary role in this process. Although neutrophils are potent producers of a wide array of proteolytic substances and are present in sunburned skin, their contribution to the pathophysiology of photoageing has been described only in murine studies. OBJECTIVES: To determine the role of neutrophils in photoageing of human skin. METHODS: Healthy white-skinned volunteers were recruited and their sun-protected buttock skin was exposed to solar-simulated radiation (SSR) in dose-response and time-course studies. Punch biopsies were taken and the influx of neutrophils and the expression of neutrophil elastase and MMPs was studied using immunohistochemical techniques and in situ zymography. RESULTS: Neutrophil elastase and MMPs were detected only in skin irradiated with erythemogenic doses (> or = 1 minimal erythema doses) of SSR. Immunohistochemical double staining demonstrated neutrophils to be the major source of MMP-1, MMP-8 and MMP-9. In situ zymography showed elastase, collagenase and gelatinase enzyme activity in those cells. CONCLUSIONS: Our study suggests that neutrophils participate in the process of photoageing of human skin as they infiltrate the skin and release enzymatically active elastase (neutrophil elastase), collagenase (MMP-1) and gelatinase (MMP-9).
BACKGROUND: The pathophysiology of photoageing of the skin has been studied extensively. Matrix metalloproteinases (MMPs) originating from keratinocytes and fibroblasts are thought to play a primary role in this process. Although neutrophils are potent producers of a wide array of proteolytic substances and are present in sunburned skin, their contribution to the pathophysiology of photoageing has been described only in murine studies. OBJECTIVES: To determine the role of neutrophils in photoageing of human skin. METHODS: Healthy white-skinned volunteers were recruited and their sun-protected buttock skin was exposed to solar-simulated radiation (SSR) in dose-response and time-course studies. Punch biopsies were taken and the influx of neutrophils and the expression of neutrophil elastase and MMPs was studied using immunohistochemical techniques and in situ zymography. RESULTS:Neutrophil elastase and MMPs were detected only in skin irradiated with erythemogenic doses (> or = 1 minimal erythema doses) of SSR. Immunohistochemical double staining demonstrated neutrophils to be the major source of MMP-1, MMP-8 and MMP-9. In situ zymography showed elastase, collagenase and gelatinase enzyme activity in those cells. CONCLUSIONS: Our study suggests that neutrophils participate in the process of photoageing of human skin as they infiltrate the skin and release enzymatically active elastase (neutrophil elastase), collagenase (MMP-1) and gelatinase (MMP-9).
Authors: C Olave; N Morales; B Uberti; C Henriquez; J Sarmiento; A Ortloff; H Folch; G Moran Journal: Vet Res Commun Date: 2018-01-02 Impact factor: 2.459
Authors: Ivana Binic; Viktor Lazarevic; Milanka Ljubenovic; Jelena Mojsa; Dusan Sokolovic Journal: Evid Based Complement Alternat Med Date: 2013-01-29 Impact factor: 2.629
Authors: Hannah M Cheeseman; Ann M Carias; Abbey B Evans; Natalia J Olejniczak; Paul Ziprin; Deborah F L King; Thomas J Hope; Robin J Shattock Journal: PLoS One Date: 2016-05-10 Impact factor: 3.240