BACKGROUND: Cutaneous wound healing is relatively slow in patients with diabetes. OBJECTIVES: To test the hypothesis that this defect in healing of wounds in patients with diabetes results from dysfunction of skin fibroblasts and epidermal keratinocytes and that this dysfunction is related to disrupted intracellular glutathione (GSH) homeostasis. METHODS: We investigated the effects of esterified GSH on the contraction of fibroblasts in a fibroblast-populated collagen lattice and on keratinocyte apoptosis. RESULTS: High glucose medium (hyperglycaemia) reduced the contraction ability of fibroblasts (P < 0.05). The normalization of glucose medium concentrations for hyperglycaemic fibroblasts did not restore the contraction capacity. The percentage of apoptotic keratinocytes was statistically higher in hyperglycaemic cells (P < 0.05). GSH media concentrations ranging from 0.1 to 100 micromol L(-1) restored the ability of hyperglycaemic fibroblasts to contract the gels in a concentration-dependent manner. Primary human keratinocytes grown in hyperglycaemic medium were more susceptible to apoptosis, and treatment with esterified GSH rescued the keratinocytes from apoptosis. CONCLUSIONS: These data suggest that intracellular GSH can normalize skin cell functions disrupted by in vitro cell growth under hyperglycaemic conditions.
BACKGROUND: Cutaneous wound healing is relatively slow in patients with diabetes. OBJECTIVES: To test the hypothesis that this defect in healing of wounds in patients with diabetes results from dysfunction of skin fibroblasts and epidermal keratinocytes and that this dysfunction is related to disrupted intracellular glutathione (GSH) homeostasis. METHODS: We investigated the effects of esterified GSH on the contraction of fibroblasts in a fibroblast-populated collagen lattice and on keratinocyte apoptosis. RESULTS: High glucose medium (hyperglycaemia) reduced the contraction ability of fibroblasts (P < 0.05). The normalization of glucose medium concentrations for hyperglycaemic fibroblasts did not restore the contraction capacity. The percentage of apoptotic keratinocytes was statistically higher in hyperglycaemic cells (P < 0.05). GSH media concentrations ranging from 0.1 to 100 micromol L(-1) restored the ability of hyperglycaemic fibroblasts to contract the gels in a concentration-dependent manner. Primary human keratinocytes grown in hyperglycaemic medium were more susceptible to apoptosis, and treatment with esterified GSH rescued the keratinocytes from apoptosis. CONCLUSIONS: These data suggest that intracellular GSH can normalize skin cell functions disrupted by in vitro cell growth under hyperglycaemic conditions.
Authors: Mohammad Esmaeelinejad; Mohammad Bayat; Hasan Darbandi; Mehrnoush Bayat; Nariman Mosaffa Journal: Lasers Med Sci Date: 2013-03-02 Impact factor: 3.161
Authors: Farzane Hendudari; Abbas Piryaei; Seyedeh-Nafiseh Hassani; Hasan Darbandi; Mohammad Bayat Journal: Lasers Med Sci Date: 2016-03-16 Impact factor: 3.161
Authors: Venkata P Mantripragada; Ryan Kaplevatsky; Wes A Bova; Cynthia Boehm; Nancy A Obuchowski; Ronald J Midura; George F Muschler Journal: Cartilage Date: 2020-02-26 Impact factor: 3.117