Guolin Li1, Li Li, Dazhong Yin. 1. Laboratory of Aging Biochemistry, College of Life Sciences, Hunan Normal University, Changsha, Hunan, P.R. China.
Abstract
OBJECTIVES: A purpose of this study is to determine whether melatonin, a novel antioxidant, can interact with malondialdehyde in an in vitro condition. METHODS: The reaction kinetics and thermodynamics of this reaction have been performed and a new product has been identified using liquid chromatography onlining with mass spectrometry. RESULTS: A new product generated with an interaction of melatonin and malondialdehyde has been identified to be a three-ring structure with a molecular weight 286, which has the specific wavelength absorption with the maximum at 345 nm. The reaction was completed within a few minutes and the product was proportional to the concentrations of the reactants. Based on its molecular weight and the deduced structure of the new product, we speculate that this reaction occurs between one melatonin molecule and one malondialdehyde and to form a 3 ring conjugated adduct. CONCLUSION: This is the first report to show that melatonin directly interacts with malondialdehyde to form a new product. The findings suggest that melatonin may detoxify unsaturated carbonyls and protect against cellular damage induced by oxidative stress.
OBJECTIVES: A purpose of this study is to determine whether melatonin, a novel antioxidant, can interact with malondialdehyde in an in vitro condition. METHODS: The reaction kinetics and thermodynamics of this reaction have been performed and a new product has been identified using liquid chromatography onlining with mass spectrometry. RESULTS: A new product generated with an interaction of melatonin and malondialdehyde has been identified to be a three-ring structure with a molecular weight 286, which has the specific wavelength absorption with the maximum at 345 nm. The reaction was completed within a few minutes and the product was proportional to the concentrations of the reactants. Based on its molecular weight and the deduced structure of the new product, we speculate that this reaction occurs between one melatonin molecule and one malondialdehyde and to form a 3 ring conjugated adduct. CONCLUSION: This is the first report to show that melatonin directly interacts with malondialdehyde to form a new product. The findings suggest that melatonin may detoxify unsaturated carbonyls and protect against cellular damage induced by oxidative stress.
Authors: Guolin Li; Yaqin Chen; Hui Hu; Li Liu; Xiaofei Hu; Jun Wang; Wang Shi; Dazhong Yin Journal: Rejuvenation Res Date: 2012-04-24 Impact factor: 4.663
Authors: Jolanta Czerwińska; Małgorzata Nowak; Patrycja Wojtczak; Dorota Dziuban-Lech; Jarosław M Cieśla; Daria Kołata; Beata Gajewska; Anna Barańczyk-Kuźma; Andria R Robinson; Hillary L Shane; Siobhán Q Gregg; Lora H Rigatti; Matthew J Yousefzadeh; Aditi U Gurkar; Sara J McGowan; Konrad Kosicki; Małgorzata Bednarek; Ewelina Zarakowska; Daniel Gackowski; Ryszard Oliński; Elżbieta Speina; Laura J Niedernhofer; Barbara Tudek Journal: Free Radic Biol Med Date: 2018-06-01 Impact factor: 7.376