| Literature DB >> 1572537 |
Y Eshita1, T Urakawa, H Hirumi, W R Fish, P A Majiwa.
Abstract
A complementary DNA expression library in phage lambda gt11 was synthesized using mRNA from in vitro-produced metacyclic forms of a clone of Trypanosoma (Nannomonas) congolense. The unamplified library was screened with antiserum from a goat immune to infection with metacyclic (m)-forms of T. congolense ILRAD Nannomonas antigen repertoire 2(ILNaR2). Of the 100 antiserum-reactive phage clones identified, 22 were analyzed further: 21 of the clones contained overlapping portions of a single transcript, while one other contained a different transcript. Northern blot analyses indicated that the sequences contained in the clones were transcribed only by m-forms of ILNaR2. Immunological and sequence analyses indicated that the two different cloned sequences encode m-form-specific variable surface glycoproteins.Entities:
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Year: 1992 PMID： 1572537 DOI： 10.1016/0378-1119(92)90389-7
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688