OBJECTIVE AND DESIGN: Chronic obstructive pulmonary disease (COPD) is characterized by an inflammatory process and airway remodeling involving matrix metalloproteinases (MMPs). Thus, we analyzed the expression and release of MMP-12 (macrophage metalloelastase) in bronchoalveolar lavage (BAL) and lung tissue from COPD patients and control subjects. METHODS: Immunocytochemistry and immunochemistry were performed to analyze the expression of MMP-12 in BAL cells and bronchial biopsies. Western blotting was used for the evaluation of MMP-12 in BAL fluids. RESULTS: The number of MMP-12 expressing macrophages together with the staining intensity was higher in BAL samples from COPD patients than in controls subjects. Similar results were noted in bronchial biopsies with higher MMP-12 expression in COPD subjects than in controls. Enhanced MMP-12 level was also observed in BAL fluids from patient with COPD in comparison to control subjects. CONCLUSION: This study demonstrated that COPD patients produce greater quantities of MMP-12 than controls, which may be a critical step in the pathogenesis of COPD and emphysema.
OBJECTIVE AND DESIGN:Chronic obstructive pulmonary disease (COPD) is characterized by an inflammatory process and airway remodeling involving matrix metalloproteinases (MMPs). Thus, we analyzed the expression and release of MMP-12 (macrophage metalloelastase) in bronchoalveolar lavage (BAL) and lung tissue from COPDpatients and control subjects. METHODS: Immunocytochemistry and immunochemistry were performed to analyze the expression of MMP-12 in BAL cells and bronchial biopsies. Western blotting was used for the evaluation of MMP-12 in BAL fluids. RESULTS: The number of MMP-12 expressing macrophages together with the staining intensity was higher in BAL samples from COPDpatients than in controls subjects. Similar results were noted in bronchial biopsies with higher MMP-12 expression in COPD subjects than in controls. Enhanced MMP-12 level was also observed in BAL fluids from patient with COPD in comparison to control subjects. CONCLUSION: This study demonstrated that COPDpatients produce greater quantities of MMP-12 than controls, which may be a critical step in the pathogenesis of COPD and emphysema.
Authors: I K Demedts; A Morel-Montero; S Lebecque; Y Pacheco; D Cataldo; G F Joos; R A Pauwels; G G Brusselle Journal: Thorax Date: 2005-11-24 Impact factor: 9.139
Authors: Michael T Borchers; Scott C Wesselkamper; Nathaniel L Harris; Hitesh Deshmukh; Erin Beckman; Mark Vitucci; Jay W Tichelaar; George D Leikauf Journal: Exp Mol Pathol Date: 2007-09-26 Impact factor: 3.362
Authors: Joanna L Richens; Richard A Urbanowicz; Elizabeth A M Lunt; Rebecca Metcalf; Jonathan Corne; Lucy Fairclough; Paul O'Shea Journal: Respir Res Date: 2009-04-22
Authors: Imran Haq; Sally Chappell; Simon R Johnson; Juzer Lotya; Leslie Daly; Kevin Morgan; Tamar Guetta-Baranes; Josep Roca; Roberto Rabinovich; Ann B Millar; Seamas C Donnelly; Vera Keatings; William MacNee; Jan Stolk; Pieter S Hiemstra; Massimo Miniati; Simonetta Monti; Clare M O'Connor; Noor Kalsheker Journal: BMC Med Genet Date: 2010-01-15 Impact factor: 2.103