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Literature DB >> 15722157

Removal of contaminating DNA from commercial nucleic acid extraction kit reagents.

Tamimount Mohammadi¹, Henk W Reesink, Christina M J E Vandenbroucke-Grauls, Paul H M Savelkoul.

Abstract

Due to contamination of DNA extraction reagents, false-positive results can occur when applying broad-range real-time PCR based on bacterial 16S rDNA. Filtration of the nucleic acid extraction kit reagents with GenElute Maxiprep binding columns was effective in removing this reagent-derived contaminating DNA while the sensitivity of the assay was maintained.

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Year: 2004 PMID： 15722157 DOI： 10.1016/j.mimet.2004.11.018

Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363

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Cited

21 in total

Review 1. Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

Authors: Geraint B Rogers; Kenneth D Bruce
Journal: Mol Diagn Ther Date: 2010-12-01 Impact factor: 4.074

2. Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

Authors: Maneesh R Pingle; Kathleen Granger; Philip Feinberg; Rebecca Shatsky; Bram Sterling; Mark Rundell; Eric Spitzer; Davise Larone; Linnie Golightly; Francis Barany
Journal: J Clin Microbiol Date: 2007-04-11 Impact factor: 5.948

Removal of contaminating DNA from commercial nucleic acid extraction kit reagents.

Review 1. Next-generation sequencing in the analysis of human microbiota: essential considerations for clinical application.

2. Multiplexed identification of blood-borne bacterial pathogens by use of a novel 16S rRNA gene PCR-ligase detection reaction-capillary electrophoresis assay.

3. Implementation of Bacterial Detection Methods into Blood Donor Screening - Overview of Different Technologies.

4. Tick Microbiome Characterization by Next-Generation 16S rRNA Amplicon Sequencing.

5. Application of next generation sequencing technology on contamination monitoring in microbiology laboratory.

6. Increasing Reproducibility in Oral Microbiome Research.

Review 7. Intricacies of assessing the human microbiome in epidemiologic studies.

8. Longitudinal profiling of the burn patient cutaneous and gastrointestinal microbiota: a pilot study.

9. Environmentally determined differences in the murine lung microbiota and their relation to alveolar architecture.

10. Optimum DNA Extraction Methods for Edible Bird's Nest Identification Using Simple Additive Weighting Technique.