Aging affects choroidal proteins involved in CSF production in Sprague-Dawley rats.

Aging is currently associated with progressive declines of cerebral functions. From these, a decreased resistance to dehydration suggested alteration in choroidal control of brain homeostasis and reduced cerebrospinal fluid (CSF) production in old subjects. In the present study, choroid plexuses of 20-month old Sprague-Dawley rats were compared with those of 3- and 10-month old rats. Using ultrastructure analysis and immunodetection of ezrin, a protein associating cytoskeleton to membranes, we showed that progressive loss of microvilli and strong decrease in apical ezrin are evident in 20-month old rats. Using immunolabeling and confocal microscopy, we found reduction in expression of two choroidal proteins, carbonic anhydrase II and aquaporin 1, involved in CSF secretion. In addition, we confirmed previous studies indicating that choroidal Na,K-ATPase decreased with age. In situ hybridization analyses showed that mRNA levels for Na,K-ATPase and aquaporin 1 were significantly lowered in choroid plexus of old rats. These findings are consistent with a reduced secretory activity in choroid plexus and suggest that massive disorders could affect choroidal CSF production in aged rats.