Literature DB >> 15715951

Identification of an antifungal chitinase from a potential biocontrol agent, Bacillus cereus 28-9.

Chien-Jui Huang1, Tang-Kai Wang, Shu-Chun Chung, Chao-Ying Chen.   

Abstract

Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This bacterium exhibited biocontrol potential on Botrytis leaf blight of lily as demonstrated by a detached leaf assay and dual culture assay. At least two chitinases (ChiCW and ChiCH) were excreted by B. cereus 28-9. The ChiCW-encoding gene was cloned and moderately expressed in Escherichia coli DH5alpha. Near homogenous ChiCW was obtained from the periplasmic fraction of E. coli cells harboring chiCW by a purification procedure. An in vitro assay showed that the purified ChiCW had inhibitory activity on conidial germination of Botrytis elliptica, a major fungal pathogen of lily leaf blight.

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Year:  2005        PMID： 15715951     DOI： 10.5483/bmbrep.2005.38.1.082

Source DB:  PubMed          Journal:  J Biochem Mol Biol        ISSN： 1225-8687


  17 in total

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