OBJECTIVE: To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells. METHOD: MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused. RESULT: Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased. CONCLUSION: The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
OBJECTIVE: To study the mechanisms of pseudolaric acid B-induced apoptosis on A375-S2 cells. METHOD:MTT, fluorescence microscope observation, DNA agarose gel electrophoresis and Western blot analysis wereused. RESULT: Pseudolaric acid Binduces A375-S2 cell apoptosis in a time and dose-dependent manner. Apoptotic bodies and DNA ladder were observed in 5 micromol x L(-1) pseudolaric acid B-treated A375-S2 cells for 36 h. The expression of Bcl-2, Bcl-xL and ICAD was reduced time dependently, whereas the expression of Bax was increased. CONCLUSION: The major cause of pseudolaric acid B induced cytotoxicity on A375-S2 cells was apoptosis. Mitochondria proteins and ICAD might be involved in the apoptotic pathways of pseudolaric acid B-treated A375-S2 cells.
Authors: Guoyi Ma; Li Chong; Xing-Cong Li; Ikhlas A Khan; Larry A Walker; Shabana I Khan Journal: J Cancer Res Clin Oncol Date: 2010-02-07 Impact factor: 4.553