[Determination of verapamil in dog serum and tissues by reversed-phase high performance liquid chromatography].

A method for determination of verapamil (VRPM) concentrations in dog serum and tissues was established. The study was carried out on a high performance liquid chromatograph with a reversed-phase C18 column, a mobile phase of acetate-methanol-triethylamine (40:60:1, v/v) at 1.0 mL/min, and UV detection at 228 nm. Diazepam was used as the internal standard. The detection limits of VRPM concentrations in serum and tissues were determined to be 50 microg/L and 50 ng/g, respectively. Good linearities were observed for VRPM in serum and in liver tissue (r > 0.999). The precision of the method was good and the recoveries of VRPM were higher than 90.80%. This method is rapid, accurate and convenient for determination of VRPM concentrations in serum and tissues.