BACKGROUND AND OBJECTIVES: Karyotype is one of the most important prognostic factors in acute myeloid leukemia (AML). DESIGN AND METHODS: To assess the diagnostic value of molecular cytogenetics in AML patients older than 60 years, we compared the results of chromosome banding with those of fluorescence in situ hybridization (FISH) applying a comprehensive DNA-probe set for the detection of the most relevant AML-associated chromosome aberrations in a prospective series of 283 patients registered for the multicenter treatment trial AML HD98-B. RESULTS: Four cases of inv(16)/t(16;16) and 2 cases of t(11q23) were only detected by FISH. Molecular cytogenetic analysis was also more sensitive for the detection of genomic imbalances, in particular 7q-, +11q, 17p-, and 20q-, but virtually all cases of aneuploidy or deletions that were missed on banding analysis were identified in patients without assessable metaphases, in patients with normal karyotypes but poor chromosome morphology, in patients with a leukemia-specific balanced rearrangement, or in patients with complex karyotypes. INTERPRETATION AND CONCLUSIONS: Our results support the use of FISH as a complementary method for the detection of inv(16)/t(16;16) and t(11q23) in all older AML patients eligible for intensive therapy. Molecular cytogenetics should also be considered in cases with insufficient yields of metaphase cells, poor chromosome morphology, or both. Routine screening for chromosomal imbalances by FISH does not improve cytogenetic risk assessment in patients with adequate pretreatment karyotype information.
BACKGROUND AND OBJECTIVES: Karyotype is one of the most important prognostic factors in acute myeloid leukemia (AML). DESIGN AND METHODS: To assess the diagnostic value of molecular cytogenetics in AMLpatients older than 60 years, we compared the results of chromosome banding with those of fluorescence in situ hybridization (FISH) applying a comprehensive DNA-probe set for the detection of the most relevant AML-associated chromosome aberrations in a prospective series of 283 patients registered for the multicenter treatment trial AML HD98-B. RESULTS: Four cases of inv(16)/t(16;16) and 2 cases of t(11q23) were only detected by FISH. Molecular cytogenetic analysis was also more sensitive for the detection of genomic imbalances, in particular 7q-, +11q, 17p-, and 20q-, but virtually all cases of aneuploidy or deletions that were missed on banding analysis were identified in patients without assessable metaphases, in patients with normal karyotypes but poor chromosome morphology, in patients with a leukemia-specific balanced rearrangement, or in patients with complex karyotypes. INTERPRETATION AND CONCLUSIONS: Our results support the use of FISH as a complementary method for the detection of inv(16)/t(16;16) and t(11q23) in all older AMLpatients eligible for intensive therapy. Molecular cytogenetics should also be considered in cases with insufficient yields of metaphase cells, poor chromosome morphology, or both. Routine screening for chromosomal imbalances by FISH does not improve cytogenetic risk assessment in patients with adequate pretreatment karyotype information.
Authors: Sylvain Mareschal; Anna Palau; Johan Lindberg; Philippe Ruminy; Christer Nilsson; Sofia Bengtzén; Marie Engvall; Anna Eriksson; Anne Neddermeyer; Vinciane Marchand; Monika Jansson; My Björklund; Fabrice Jardin; Mattias Rantalainen; Andreas Lennartsson; Lucia Cavelier; Henrik Grönberg; Sören Lehmann Journal: Blood Adv Date: 2021-02-23
Authors: Richard F Schlenk; Konstanze Döhner; Michael Kneba; Katharina Götze; Frank Hartmann; Francesco Del Valle; Heinz Kirchen; Elisabeth Koller; Jörg T Fischer; Lars Bullinger; Marianne Habdank; Daniela Späth; Silja Groner; Bernhard Krebs; Sabine Kayser; Andrea Corbacioglu; Andreas Anhalt; Axel Benner; Stefan Fröhling; Hartmut Döhner Journal: Haematologica Date: 2008-12-04 Impact factor: 9.941