| Literature DB >> 15708822 |
Ana Rita M Nardi1, Márcia R Salvadori, Lia T Coswig, Maria Sílvia V Gatti, Domingos S Leite, Geórgio F Valadares, Manoel Garcia Neto, Rubén P Shocken-Iturrino, Jesús E Blanco, Tomomasa Yano.
Abstract
The culture supernatant of Escherichia coli, isolated from ostriches with diarrhea in Brazil, caused elongation in Vero cell, rounding in Chinese hamster ovary (CHO) cells and a cytoplasmic vacuolation in ostrich embryo fibroblasts (OEF), but it was not cytotoxic for chicken embryo fibroblasts (CEF). These effects were not neutralized by antiserum to cholera toxin. Polymerase chain reaction assays showed that the ostrich E. coli contained the gene encoding (eltII-A), but not those for type 1 heat-labile enterotoxin (eltA), heat-stable enterotoxins (estA, estB), verocytotoxins (stx-I, stx-II), or cytotoxic necrotizing factors (cnf 1, cnf 2). All isolates belonged to serotype O15:H8. The enteropathogenic relevance of LT-II in ostrich diarrhea remains undetermined.Entities:
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Year: 2004 PMID： 15708822 DOI： 10.1016/j.vetmic.2004.11.005
Source DB: PubMed Journal: Vet Microbiol ISSN： 0378-1135 Impact factor: 3.293