Qing H Meng1, Donna Gauthier. 1. Department of Pathology and Molecular Medicine, McMaster University, Ontario, Canada L8N 4A6. qing.meng@usask.ca
Abstract
OBJECTIVES: To develop a HPLC method for the determination of citalopram (CIT) and desmethylcitalopram (DCIT). METHODS: Solid-phase extraction followed by fluorescence detection was used to measure CIT and DCIT in deproteinized plasma. RESULTS: The extraction recovery for both analytes was 104 +/- 3%. The calibration was linear over the concentration range of 12-1600 ng/mL for CIT and 6-800 ng/mL for DCIT. The within-run CVs were 2.5% for CIT (400 ng/mL) and 2.9% for DCIT (200 ng/mL) and the between-run CVs were 5.2% for CIT and 2.9% for DCIT, respectively. With low concentrations of CIT (50 ng/mL) and DCIT (25 ng/mL), the within-run CVs were 3.1% and 1.1% and the between-run CVs were 7.4% and 8.8%, respectively. The lower limit of quantification was 12 ng/mL for CIT and 6 ng/mL for DCIT. CONCLUSIONS: This method allows for the simultaneous determination of CIT and DCIT in plasma at therapeutic and toxic drug concentrations.
OBJECTIVES: To develop a HPLC method for the determination of citalopram (CIT) and desmethylcitalopram (DCIT). METHODS: Solid-phase extraction followed by fluorescence detection was used to measure CIT and DCIT in deproteinized plasma. RESULTS: The extraction recovery for both analytes was 104 +/- 3%. The calibration was linear over the concentration range of 12-1600 ng/mL for CIT and 6-800 ng/mL for DCIT. The within-run CVs were 2.5% for CIT (400 ng/mL) and 2.9% for DCIT (200 ng/mL) and the between-run CVs were 5.2% for CIT and 2.9% for DCIT, respectively. With low concentrations of CIT (50 ng/mL) and DCIT (25 ng/mL), the within-run CVs were 3.1% and 1.1% and the between-run CVs were 7.4% and 8.8%, respectively. The lower limit of quantification was 12 ng/mL for CIT and 6 ng/mL for DCIT. CONCLUSIONS: This method allows for the simultaneous determination of CIT and DCIT in plasma at therapeutic and toxic drug concentrations.
Authors: Juan C Velasquez; Qiuying Zhao; Yen Chan; Ligia C M Galindo; Christelle Simasotchi; Dan Wu; Zhipeng Hou; Skyla M Herod; Tim F Oberlander; Sophie Gil; Thierry Fournier; Irina Burd; Anne M Andrews; Alexandre Bonnin Journal: ACS Chem Neurosci Date: 2019-06-24 Impact factor: 4.418