| Literature DB >> 15708310 |
Zia Sayed1, Frederick Harris, David A Phoenix.
Abstract
"Comet assay" showed light activated (3.15 Jcm-2 over 30 min) phenothiazinium based photosensitisers (PhBPs) to induce photo-damage of Staphylococcus aureus DNA, as indicated by DNA "tails" between 80 and 120 microm. In general, PhBPs exhibited significant singlet oxygen yields (Phi(DeltaPhBP)>0.7), suggesting the use of type II mechanisms of photo-oxidation. However, the photodynamic action of PhBPs on DNA showed generally insignificant production of 7,8-dihydro-8-oxo-2'-deoxyguanosine, normally a major product of type II DNA photo-oxidation. These combined results show DNA to be a major site of action of PhBPs and suggest that this action may involve type II attack on a nucleoside(s) other than guanosine.Entities:
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Year: 2005 PMID： 15708310 DOI： 10.1016/j.femsim.2004.09.002
Source DB: PubMed Journal: FEMS Immunol Med Microbiol ISSN： 0928-8244