OBJECTIVE: To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills. METHOD: Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm. RESULT: Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively. CONCLUSION: This method can be used for quality control of Yixintong pills.
OBJECTIVE: To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills. METHOD: Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm. RESULT: Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively. CONCLUSION: This method can be used for quality control of Yixintong pills.
Authors: Xi Xiang Ying; Fei Wang; Zhong Zhe Cheng; Wen Jie Zhang; Hai Bo Li; Yang Du; Xun Liu; Si Yuan Wang; Ting Guo Kang Journal: Eur J Drug Metab Pharmacokinet Date: 2011-11-06 Impact factor: 2.441