Continuous production of neo-fructooligosaccharides by immobilization of whole cells of Penicillium citrinum.

To produce neo-fructooligosaccharides (neo-FOSs) in a 500 ml continuous packed-bed reactor using whole cell immobilization of Penicillium citrinum KCCM 11663, the optimum reaction conditions were 50 degrees C, pH 6 with 600 g sucrose l-1 being fed as substrate at 1.3 ml min-1 . Under these conditions, the maximum neo-FOSs production was 49 g l-1. In a packed-bed reactor, continuous production of neo-FOSs was possible for 50 d indicating a potential for industrial production.