Comparison of the retinal structure and function in four bird species as a function of the time they start singing in the morning.

We postulated that the retinas of bird species that are the earlier singers are more sensitive to low light conditions than species that sing closer to sunrise. The selected species were the American Robin (Turdus migratorius) and the Hermit Thrush (Catharus guttatus) as early singers, the Common Grackle (Quiscalus quiscula) and the Mourning Dove (Zenaida macroura) which join the dawn chorus near sunrise. Scotopic electroretinogram (ERGs) intensity-response functions were obtained from anesthetized birds, following which the animals were euthanized and their retinas processed for histological analysis. Based on k values, generally considered an adequate measurement of the scotopic (rod) retinal sensitivity, all species yielded comparable night vision capabilities. However, based on the maximal (mixed rod-cone response) amplitude of the scotopic b-wave, our results indicate that robins and thrushes yield larger scotopic ERGs compared with doves and grackles, but unexpectedly do not have higher rod:cone ratios. Increased thickness of the retinal inner nuclear layer and higher numbers of ganglion cells in robins and thrushes compared with doves and grackles suggest a greater number of synaptic connections maximizing vision under low light conditions, and might support their higher retinal responses under scotopic conditions. The higher ERG V(max) of robins and thrushes might also be explained, at least in part, by the optics (i.e., their lower minimum F-numbers), resulting in brighter retinal images, rather than from higher sensitivity of their retina. Our results suggest that an early onset of dawn singing might be correlated with the retinal ability to detect the first dim crepuscular lights.