Literature DB >> 15702550

Rapid G+ count and subpopulation assessment of the intestinal bacteria in Apodemus sylvaticus and Mus musculus by flow cytometry.

M Moragues1, J Comas-Riu, J Vives-Rego.   

Abstract

We report a novel application of calcein-acetomethyl ester in flow cytometry for rapid estimation of the number of G+-bacteria in rodent feces (Apodemus sylvaticus and Mus sp.f. muridae). We also use the combined application of flow cytometry and Syto-13 or Sypro Orange staining to count rapidly the total bacterial population and to describe bacterial subpopulations in the intestine.

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Year:  2004        PMID： 15702550     DOI： 10.1007/bf02931538

Source DB:  PubMed          Journal:  Folia Microbiol (Praha)        ISSN： 0015-5632            Impact factor:   2.099


  8 in total

Review 1.  A molecular view of the intestinal ecosystem.

Authors:  E E Vaughan; F Schut; H G Heilig; E G Zoetendal; W M de Vos; A D Akkermans
Journal:  Curr Issues Intest Microbiol       Date:  2000-03

Review 2.  Current and future applications of flow cytometry in aquatic microbiology.

Authors:  J Vives-Rego; P Lebaron; G Nebe-von Caron
Journal:  FEMS Microbiol Rev       Date:  2000-10       Impact factor: 16.408

3.  Statistical analysis and biological interpretation of the flow cytometric heterogeneity observed in bacterial axenic cultures.

Authors:  J Vives-Rego; O Resina; J Comas; G Loren; O Julià
Journal:  J Microbiol Methods       Date:  2003-04       Impact factor: 2.363

4.  Assessment of the effects of gramicidin, formaldehyde, and surfactants on Escherichia coli by flow cytometry using nucleic acid and membrane potential dyes.

Authors:  J Comas; J Vives-Rego
Journal:  Cytometry       Date:  1997-09-01

5.  Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut.

Authors:  A Suau; R Bonnet; M Sutren; J J Godon; G R Gibson; M D Collins; J Doré
Journal:  Appl Environ Microbiol       Date:  1999-11       Impact factor: 4.792

6.  Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli.

Authors:  H Berger; J Hacker; A Juarez; C Hughes; W Goebel
Journal:  J Bacteriol       Date:  1982-12       Impact factor: 3.490

7.  Involvement of RNA and DNA in the staining of Escherichia coli by SYTO 13.

Authors:  T Guindulain; J Vives-Rego
Journal:  Lett Appl Microbiol       Date:  2002       Impact factor: 2.858

8.  Use of nucleic acid dyes SYTO-13, TOTO-1, and YOYO-1 in the study of Escherichia coli and marine prokaryotic populations by flow cytometry.

Authors:  T Guindulain; J Comas; J Vives-Rego
Journal:  Appl Environ Microbiol       Date:  1997-11       Impact factor: 4.792
  8 in total
  3 in total

1.  Development of flow cytometry technique for detection of thinning of peptidoglycan layer as a result of solvent production by Clostridium pasteurianum.

Authors:  M Linhová; P Patáková; J Lipovský; P Fribert; L Paulová; M Rychtera; K Melzoch
Journal:  Folia Microbiol (Praha)       Date:  2010-08-03       Impact factor: 2.099

2.  Distribution of bifidobacteria in the gastrointestinal tract of calves.

Authors:  E Vlková; I Trojanová; V Rada
Journal:  Folia Microbiol (Praha)       Date:  2006       Impact factor: 2.099

3.  Dual channel detection of ultra low concentration of bacteria in real time by scanning FCS.

Authors:  Ilaria Altamore; Luca Lanzano; Enrico Gratton
Journal:  Meas Sci Technol       Date:  2013-06-01       Impact factor: 2.046
  3 in total

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