OBJECTIVE: This study was undertaken to develop a rabbit model of maternal exposure to Porphyromonas gingivalis and determine whether fetal or placental exposure occurs. STUDY DESIGN: Subcutaneous steel chambers were implanted in 8 New Zealand White female rabbits. On day 7 of pregnancy, 4 rabbits were inoculated through the chamber with 5 x 10 8 CFU/mL live P gingivalis , and 4 rabbits with broth (controls) and sacrificed at term. Polymerase chain reaction was used to detect P gingivalis in maternal and fetal liver and placenta. Fisher exact test was used to compare P gingivalis detection between groups. RESULTS: Among exposed does, P gingivalis was detected in 33% of the maternal livers, 49% of placentas, and 34% fetal livers compared with none from controls ( P < .001). CONCLUSION: Chronic maternal exposure to P gingivalis results in systemic dissemination, transplacental passage, and fetal exposure. This model may be useful to study placental and fetal effects of this oral pathogen and to study microbial dissemination across the placenta.
OBJECTIVE: This study was undertaken to develop a rabbit model of maternal exposure to Porphyromonas gingivalis and determine whether fetal or placental exposure occurs. STUDY DESIGN: Subcutaneous steel chambers were implanted in 8 New Zealand White female rabbits. On day 7 of pregnancy, 4 rabbits were inoculated through the chamber with 5 x 10 8 CFU/mL live P gingivalis , and 4 rabbits with broth (controls) and sacrificed at term. Polymerase chain reaction was used to detect P gingivalis in maternal and fetal liver and placenta. Fisher exact test was used to compare P gingivalis detection between groups. RESULTS: Among exposed does, P gingivalis was detected in 33% of the maternal livers, 49% of placentas, and 34% fetal livers compared with none from controls ( P < .001). CONCLUSION: Chronic maternal exposure to P gingivalis results in systemic dissemination, transplacental passage, and fetal exposure. This model may be useful to study placental and fetal effects of this oral pathogen and to study microbial dissemination across the placenta.
Authors: Roberto Romero; Jimmy Espinoza; Luís F Gonçalves; Juan Pedro Kusanovic; Lara Friel; Sonia Hassan Journal: Semin Reprod Med Date: 2007-01 Impact factor: 1.303
Authors: Morgan R Peltier; Cayce O Drobek; Geeta Bhat; George Saade; Stephen J Fortunato; Ramkumar Menon Journal: J Reprod Immunol Date: 2012-09-25 Impact factor: 4.054