Host nutrition determines blood nutrient composition and mediates parasite developmental success: Manduca sexta L. parasitized by Cotesia congregata (Say).

This investigation examined the influence of dietary protein and carbohydrate balance in a chemically defined artificial diet for Manduca sexta larvae on development of the gregarious parasite Cotesia congregata. Normal unparasitized larvae and larvae superparasitized in the fourth stadium were reared to the end of the fifth stadium on six diets, each having the same total amount of casein and sucrose but with different ratios ranging from high protein/no carbohydrate through to low protein/high carbohydrate. Levels of blood protein nitrogen and trehalose, nutrients supporting growth and development of C. congregata, varied with diet and were influenced by parasitism. Different levels of blood metabolites reflected differences in diet consumption, and the relationships between protein nitrogen and trehalose were very similar to those for protein and carbohydrate intake by parasitized and normal larvae on various diets. Dietary nutrient ratio had a significant effect on parasite burden, the numbers of parasites developing in individual host larvae and on parasite biomass. Parasites included individuals that developed and eventually emerged as second instar larvae, moulted to third instars and pupated. Many apparently mature second instar parasites, however, failed to emerge. The proportion of non-emerging individuals varied with diet, and in some cases, parasites failing to emerge were greater in number and total biomass than those that did emerge to complete development. On most diets, the mass of individual parasites was similar regardless of dietary nutrient ratio. Three dimensional models developed to demonstrate the relationships between blood protein nitrogen and trehalose levels and parasite burden and biomass established that the levels of both metabolites are important for supporting growth and development of emerged and non-emerged parasites. In the case of emerged parasites, however, the relationships are linear, and a quadratic function best describes the relationships with non-emerged parasites. Blood metabolite levels supporting the greatest parasite burden and biomass of emerged and non-emerged parasites occupy a region of two dimensional space corresponding to approximately 60-200 mg per insect of protein nitrogen and 60-100 mg per insect of trehalose. Despite the differences in the response of emerged and non-emerged parasites to host nutrition, the present results indicate that host nutrition is not the critical factor determining parasite emergence. The significance of these findings to the biology of C. congregata is discussed.