| Literature DB >> 1569575 |
V Cody1, J R Luft, W Pangborn, J Toy, A L Bognar.
Abstract
Folylpolyglutamate synthetase (FPGS) from Lactobacillus casei has been crystallized with polyethylene glycol and acetate buffer at pH 5.0. The enzyme was obtained from Escherichia coli strain SF4 harboring the L. casei FPGS chromosomal gene on a pEMBL vector (pGT3-8.1). Crystals of the enzyme were obtained which diffract to 2.6 A resolution. The crystals are monoclinic, space group P2(1), with unit cell dimensions of a = 54.07 A, b = 45.83 A, c = 84.37 A and beta = 107.92 degrees. A unit cell contains one molecule of the 43,000 Da enzyme per asymmetric unit. A complete X-ray data set on the native crystals has been collected.Entities:
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Year: 1992 PMID： 1569575 DOI： 10.1016/0022-2836(92)90480-8
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469