| Literature DB >> 1569568 |
M R Walter1, W J Cook, S E Ealick, T L Nagabhushan, P P Trotta, C E Bugg.
Abstract
The crystal structure of recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) has been determined at 2.8 A resolution using multiple isomorphous replacement techniques. There are two molecules in the crystallographic asymmetric unit, which are related by an approximate non-crystallographic 2-fold axis. The overall structure is highly compact and globular with a predominantly hydrophobic core. The main structural feature of rhGM-CSF is a four alpha-helix bundle, which represents approximately 42% of the structure. The helices are arranged in a left-handed antiparallel bundle with two overhand connections. Within the connections is a two-stranded antiparallel beta-sheet. The tertiary structure of rhGM-CSF has a topology similar to that of porcine growth factor and interferon-beta. Most of the proposed critical regions for receptor binding are located on a continuous surface at one end of the molecule that includes the C terminus.Entities:
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Year: 1992 PMID： 1569568 DOI： 10.1016/0022-2836(92)90470-5
Source DB: PubMed Journal: J Mol Biol ISSN： 0022-2836 Impact factor: 5.469