| Literature DB >> 15694378 |
Keiichi Ikeda1, Katsuyoshi Tojo, Chikara Otsubo, Takashi Udagawa, Kensuke Kumazawa, Masahiro Ishikawa, Goro Tokudome, Tatsuo Hosoya, Naoko Tajima, William C Claycomb, Kazuwa Nakao, Masahiro Kawamura.
Abstract
Some reports showed that serotonergic system might have existed and that 5-hydroxytryptamine (5-HT) was detected in the hamster heart. The source of 5-HT in the heart, however, remains to be fully elucidated. So the present study was designed to define serotonergic system and to clarify which cell could produce 5-HT in the heart. As a result, 5-HT was detected in homogenates of HL-1 cardiomyocytes by high performance liquid chromatography with fluorescence detection, but not in those of neonatal rat non-cardiomyocytes (NMCs). And TPH and AADC mRNAs were expressed in HL-1 cardiomyocytes and neonatal rat cardiomyocytes (MCs), not in NMCs. mRNAs of 5-HT(2A) receptor were detected in both MCs and NMCs, and those of 5-HT(2B) receptor in NMCs. These findings definitively demonstrate that 5-HT is secreted from the myocytes of the heart and strongly implied that 5-HT might play a certain role in cardiac physiology.Entities:
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Year: 2005 PMID： 15694378 DOI： 10.1016/j.bbrc.2005.01.018
Source DB: PubMed Journal: Biochem Biophys Res Commun ISSN： 0006-291X Impact factor: 3.575