QUESTION: Is it possible to stimulate osteoconduction and osteogenesis to improve bone formation in critical-size defects in order to avoid bone grafting? MATERIAL AND METHODS: Full thickness, critical-sized defects were created in the anterior mandible of 16 adult mini-pigs. The defects were filled with a new bioactive matrix (60% hydroxyapatite and 40% beta-tricalciumphosphate), produced by an innovative low temperature sol-gel-process (120 degrees C). The biomatrix was tested alone and in combination with cultured autologous osteoblasts. In a control group, periosteum was the only bone producing source. Five weeks postoperatively, the animals were sacrificed and the defects analysed macroscopically, histologically and radiographically. RESULTS: The highest rate of new bone formation was in the biomatrix group without osteoblasts (73% of the former defect). The biomatrix was degraded at the same speed as new bone was laid down. In the control group, bone formation of only 59% was observed. Additional transplantation of autologous osteoblasts in combination with the biomatrix did not result in more bone production than in the control group(!). CONCLUSION: This new bioactive calciumphosphate matrix seems to be a promising bone replacement material.
QUESTION: Is it possible to stimulate osteoconduction and osteogenesis to improve bone formation in critical-size defects in order to avoid bone grafting? MATERIAL AND METHODS: Full thickness, critical-sized defects were created in the anterior mandible of 16 adult mini-pigs. The defects were filled with a new bioactive matrix (60% hydroxyapatite and 40% beta-tricalciumphosphate), produced by an innovative low temperature sol-gel-process (120 degrees C). The biomatrix was tested alone and in combination with cultured autologous osteoblasts. In a control group, periosteum was the only bone producing source. Five weeks postoperatively, the animals were sacrificed and the defects analysed macroscopically, histologically and radiographically. RESULTS: The highest rate of new bone formation was in the biomatrix group without osteoblasts (73% of the former defect). The biomatrix was degraded at the same speed as new bone was laid down. In the control group, bone formation of only 59% was observed. Additional transplantation of autologous osteoblasts in combination with the biomatrix did not result in more bone production than in the control group(!). CONCLUSION: This new bioactive calciumphosphate matrix seems to be a promising bone replacement material.
Authors: Christoph Harms; Kai Helms; Tibor Taschner; Ioannis Stratos; Anita Ignatius; Thomas Gerber; Solvig Lenz; Stefan Rammelt; Brigitte Vollmar; Thomas Mittlmeier Journal: Int J Nanomedicine Date: 2012-06-15
Authors: Florian Andreas Probst; Riham Fliefel; Egon Burian; Monika Probst; Matthias Eddicks; Matthias Cornelsen; Christina Riedl; Hermann Seitz; Attila Aszódi; Matthias Schieker; Sven Otto Journal: Sci Rep Date: 2020-02-06 Impact factor: 4.379
Authors: Hossein Nejadnik; Tobias D Henning; Thuy Do; Elizabeth J Sutton; Frederick Baehner; Andrew Horvai; Barbara Sennino; Donald McDonald; Reinhard Meier; Bernd Misselwitz; Thomas M Link; Heike E Daldrup-Link Journal: PLoS One Date: 2012-12-12 Impact factor: 3.240