OBJECTIVE: Scavenger receptor class B type I (SR-BI)/apolipoprotein E (apoE) double knockout (dKO) mice exhibit many features of human coronary heart disease (CHD), including occlusive coronary atherosclerosis, cardiac hypertrophy, myocardial infarctions, and premature death. Here we determined the influence of B and T lymphocytes, which can contribute to atherosclerosis, ischemia-reperfusion injury, and cardiomyocyte death, on pathology in dKO mice. METHOD AND RESULTS: The lymphocyte-deficient SR-BI/apoE/recombination activating gene 2 (RAG2) triple knockout mice and corresponding dKO controls generated for this study exhibited essentially identical lipid-rich coronary occlusions, myocardial infarctions, cardiac dysfunction, and premature death (average lifespans 41.6+/-0.6 and 42.0+/-0.5 days, respectively). CONCLUSIONS: B and T lymphocytes and associated immunoglobulin-mediated inflammation are not essential for the development and progression of CHD in dKO mice. Strikingly, the dKO mice bred for this study (mixed C57BL/6xSV129xBALB/c background; strain 2) compared with the previously described dKO mice (75:25 C57BL/6:SV129 background; strain 1) had a shorter mean lifespan and steeper survival curve, characteristics especially attractive for studying the effects of environmental, pharmacological, and genetic manipulations on cardiac pathophysiology.
OBJECTIVE:Scavenger receptor class B type I (SR-BI)/apolipoprotein E (apoE) double knockout (dKO) mice exhibit many features of humancoronary heart disease (CHD), including occlusive coronary atherosclerosis, cardiac hypertrophy, myocardial infarctions, and premature death. Here we determined the influence of B and T lymphocytes, which can contribute to atherosclerosis, ischemia-reperfusion injury, and cardiomyocyte death, on pathology in dKO mice. METHOD AND RESULTS: The lymphocyte-deficient SR-BI/apoE/recombination activating gene 2 (RAG2) triple knockout mice and corresponding dKO controls generated for this study exhibited essentially identical lipid-rich coronary occlusions, myocardial infarctions, cardiac dysfunction, and premature death (average lifespans 41.6+/-0.6 and 42.0+/-0.5 days, respectively). CONCLUSIONS: B and T lymphocytes and associated immunoglobulin-mediated inflammation are not essential for the development and progression of CHD in dKO mice. Strikingly, the dKO mice bred for this study (mixed C57BL/6xSV129xBALB/c background; strain 2) compared with the previously described dKO mice (75:25 C57BL/6:SV129 background; strain 1) had a shorter mean lifespan and steeper survival curve, characteristics especially attractive for studying the effects of environmental, pharmacological, and genetic manipulations on cardiac pathophysiology.
Authors: Vandana S Dole; Jana Matuskova; Eliza Vasile; Ayce Yesilaltay; Wolfgang Bergmeier; Michael Bernimoulin; Denisa D Wagner; Monty Krieger Journal: Arterioscler Thromb Vasc Biol Date: 2008-04-24 Impact factor: 8.311
Authors: Kosuke Tsukamoto; D R Mani; Jianru Shi; Songwen Zhang; Darrow E Haagensen; Fumiyuki Otsuka; Jian Guan; Jonathan D Smith; Wei Weng; Ronglih Liao; Frank D Kolodgie; Renu Virmani; Monty Krieger Journal: Proc Natl Acad Sci U S A Date: 2013-09-30 Impact factor: 11.205
Authors: Ying Pei; Xing Chen; Dina Aboutouk; Mark T Fuller; Omid Dadoo; Pei Yu; Elizabeth J White; Suleiman A Igdoura; Bernardo L Trigatti Journal: PLoS One Date: 2013-08-13 Impact factor: 3.240