Cytogenetic study of breast cancer: clinicopathologic significance of homogeneously staining regions in 84 patients.

A cytogenetic study performed on 84 primary breast cancers was conducted to detect the presence of chromosomes with homogeneously staining regions (HSRs) which are assumed to correspond to gene amplification. Homogeneously staining regions were found in 60% of the cases. To assess the prognostic significance of HSRs, clinical and pathologic data were correlated with the cytogenetic findings. Median follow-up for the 84 patients was 40 months. Differences in overall survival and disease-free survival rates between patients with HSRs (50 cases) and patients without HSRs (34 cases) were not significant. Although an excess of HSRs was found in patients considered to have poor prognostic factors (eg, young age, high histologic grade, metastatic axillary nodes, loss of hormonal receptors), no statistically significant correlation between variables could be demonstrated. The high percentage of cases with HSRs strongly suggests that they have an important biologic role, but more cases should be studied with a longer follow-up to assess their significance.