BACKGROUND: There is a lack of data in the literature concerning the identification of potential prognostic factors in ampullary adenocarcinoma. AIMS: To examine the prognostic significance of Bax, Bcl-2, and p53 protein expression and the apoptotic index in a large cohort of uniformly treated patients with radically resected ampullary cancer. METHODS: All patients with a pathological diagnosis of ampullary cancer and radical resection were evaluated. Expression analysis for p53, Bax, and Bcl-2 was performed by immunohistochemistry. Apoptotic cells were identified by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL). RESULTS: Thirty nine tumour specimens from patients with radically resected ampullary adenocarcinoma were studied. A positive significant correlation between Bax and p53 expression was found by rank correlation matrix (p < 0.001). A trend towards a positive correlation was found between the apoptotic index and p53 expression (p = 0.059). By univariate analysis, overall survival was influenced by Bax expression, p53 expression, and TUNEL staining (p = 0.001, p = 0.01, and p = 0.03, respectively). Bcl-2 expression did not influence overall survival in these patients (p = 0.55). By multivariate Cox regression analysis, the only immunohistochemical parameter that influenced overall survival was Bax expression (p = 0.020). CONCLUSIONS: These results provide evidence that apoptosis may be an important prognostic factor in patients with radically resected ampullary cancer. This study is the first to assess the clinical usefulness of Bax expression in radically resected ampullary cancer.
BACKGROUND: There is a lack of data in the literature concerning the identification of potential prognostic factors in ampullary adenocarcinoma. AIMS: To examine the prognostic significance of Bax, Bcl-2, and p53 protein expression and the apoptotic index in a large cohort of uniformly treated patients with radically resected ampullary cancer. METHODS: All patients with a pathological diagnosis of ampullary cancer and radical resection were evaluated. Expression analysis for p53, Bax, and Bcl-2 was performed by immunohistochemistry. Apoptotic cells were identified by terminal deoxynucleotidyl transferase mediated dUTP nick end labelling (TUNEL). RESULTS: Thirty nine tumour specimens from patients with radically resected ampullary adenocarcinoma were studied. A positive significant correlation between Bax and p53 expression was found by rank correlation matrix (p < 0.001). A trend towards a positive correlation was found between the apoptotic index and p53 expression (p = 0.059). By univariate analysis, overall survival was influenced by Bax expression, p53 expression, and TUNEL staining (p = 0.001, p = 0.01, and p = 0.03, respectively). Bcl-2 expression did not influence overall survival in these patients (p = 0.55). By multivariate Cox regression analysis, the only immunohistochemical parameter that influenced overall survival was Bax expression (p = 0.020). CONCLUSIONS: These results provide evidence that apoptosis may be an important prognostic factor in patients with radically resected ampullary cancer. This study is the first to assess the clinical usefulness of Bax expression in radically resected ampullary cancer.
Authors: I Sturm; H Petrowsky; R Volz; M Lorenz; S Radetzki; T Hillebrand; G Wolff; S Hauptmann; B Dörken; P T Daniel Journal: J Clin Oncol Date: 2001-04-15 Impact factor: 44.544
Authors: A Scarpa; C Di Pace; G Talamini; M Falconi; N R Lemoine; C Iacono; A Achille; A Baron; G Zamboni Journal: Gut Date: 2000-06 Impact factor: 23.059
Authors: R C van Geenen; T M van Gulik; G J Offerhaus; L T de Wit; O R Busch; H Obertop; D J Gouma Journal: Eur J Surg Oncol Date: 2001-09 Impact factor: 4.424
Authors: A M Thompson; T J Anderson; A Condie; J Prosser; U Chetty; D C Carter; H J Evans; C M Steel Journal: Int J Cancer Date: 1992-02-20 Impact factor: 7.396
Authors: Ketut Surya Negara; Ni Luh Lany Christina Prajawati; Gede Putu Surya; Suhendro Suhendro; Komang Arijana; Ketut Tunas Journal: Open Access Maced J Med Sci Date: 2018-11-20
Authors: Wenyan Zhang; Rui Duan; Jian Zhang; William K C Cheung; Xiaoge Gao; Raymond Zhang; Qing Zhang; Mengxue Wei; Gang Wang; Qian Zhang; Peng-Jin Mei; Hong-Lin Chen; Hsiangfu Kung; Marie C Lin; Zan Shen; Junnian Zheng; Longzhen Zhang; Hong Yao Journal: Br J Cancer Date: 2018-01-18 Impact factor: 7.640