OBJECTIVE: Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, but its precise mechanism of action in vivo has remained unknown. METHODS: We measured ET-1 activity by radioimmunoassay, in both plasma and synovial fluid from patients with inflammatory arthritides. RESULTS: ET-1-like immunoreactivity was found in synovial fluid, at levels severalfold higher than those in plasma. Reverse-phase high-performance liquid chromatography showed an elution profile corresponding to actual ET-1. A single class of high-affinity binding sites for ET-1 in cultured synovial cells was also detected. Furthermore, ET-1 induced mild DNA synthesis in synovial cells. CONCLUSION: Taken together, these results indicate that ET-1 might contribute to the synovial proliferation seen in inflammatory arthritides, in an autocrine/paracrine manner.
OBJECTIVE:Endothelin-1 (ET-1) is a potent vasoconstrictive peptide, but its precise mechanism of action in vivo has remained unknown. METHODS: We measured ET-1 activity by radioimmunoassay, in both plasma and synovial fluid from patients with inflammatory arthritides. RESULTS:ET-1-like immunoreactivity was found in synovial fluid, at levels severalfold higher than those in plasma. Reverse-phase high-performance liquid chromatography showed an elution profile corresponding to actual ET-1. A single class of high-affinity binding sites for ET-1 in cultured synovial cells was also detected. Furthermore, ET-1 induced mild DNA synthesis in synovial cells. CONCLUSION: Taken together, these results indicate that ET-1 might contribute to the synovial proliferation seen in inflammatory arthritides, in an autocrine/paracrine manner.
Authors: Paula B Donate; Thiago M Cunha; Waldiceu A Verri; Cristina M Junta; Flavia O Lima; Silvio M Vieira; Rafael S Peres; Karina F Bombonato-Prado; Paulo Louzada; Sergio H Ferreira; Eduardo A Donadi; Geraldo A S Passos; Fernando Q Cunha Journal: Inflamm Res Date: 2012-01-17 Impact factor: 4.575
Authors: H Sakurai; H Kohsaka; M F Liu; H Higashiyama; Y Hirata; K Kanno; I Saito; N Miyasaka Journal: J Clin Invest Date: 1995-11 Impact factor: 14.808
Authors: M Uguccioni; L Pulsatelli; B Grigolo; A Facchini; L Fasano; C Cinti; M Fabbri; G Gasbarrini; R Meliconi Journal: J Clin Pathol Date: 1995-04 Impact factor: 3.411
Authors: Karla G G Serafim; Suelen A Navarro; Ana C Zarpelon; Felipe A Pinho-Ribeiro; Victor Fattori; Thiago M Cunha; Jose C Alves-Filho; Fernando Q Cunha; Rubia Casagrande; Waldiceu A Verri Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 2015-08-06 Impact factor: 3.000