Ahmad S Al-Hiyasat1, Homa Darmani. 1. Department of Restorative Dentistry, Jordan University of Science and Technology, Irbid 22110, Jordan. hiyasat@just.edu.jo
Abstract
STATEMENT OF PROBLEM: Cytotoxicity of cast alloys depends on their composition and element release, which may be affected by recasting of the alloy. PURPOSE: This study investigated the effect of recasting on the element release and cytotoxicity of 5 base metal alloys. MATERIAL AND METHODS: The alloys tested consisted of: 2 Ni-Cr (Remanium CS and Wiron 99); a Ni-Cr with Cu (CB Soft); a Co-Cr (Wirobond C); and a Cu-base (Thermobond). Alloy specimens (disks 3 x 5 mm) were cast as follows: 100% new alloy; 50% new with 50% recast; and 100% recast (n = 6). Cytotoxicity was tested using Balb/C 3T3 fibroblasts and the MTT assay. Six replicates were tested for each alloy, and the controls consisted of 6 wells containing cells with no alloy specimens. The release of elements from the alloys into culture medium was also investigated. The data were analyzed by 2-way analysis of variance (ANOVA) followed by the Tukey Honestly Significant Difference test (family error rate =.05). RESULTS: At all 3 variations of casting, Remanium CS and Wiron 99 were the least cytotoxic, followed by Wirobond C, CB Soft, and Thermobond. ANOVA revealed significant differences between the groups (P <.001). Post hoc analysis showed that overall, Thermobond was significantly more cytotoxic than all of the other alloys tested (P <.05). For the 100% new alloy groups the cytotoxicity of Co-Cr alloy Wirobond C was similar to that of the Ni-Cr alloys Remanium CS and Wiron 99; however, when the alloys were recast, Wirobond C became significantly more cytotoxic and as toxic as the Ni-Cr alloy containing Cu (CB Soft). Moreover, recasting significantly increased the cytotoxicity of all the alloys (P <.05) and the amounts of elements released from them. CONCLUSION: The Cu content in an alloy increased its cytotoxicity level remarkably. Recasting of alloys significantly increased their cytotoxicity level. The Co-Cr alloy was more adversely affected by the recasting than the Ni-Cr alloys.
STATEMENT OF PROBLEM: Cytotoxicity of cast alloys depends on their composition and element release, which may be affected by recasting of the alloy. PURPOSE: This study investigated the effect of recasting on the element release and cytotoxicity of 5 base metalalloys. MATERIAL AND METHODS: The alloys tested consisted of: 2 Ni-Cr (Remanium CS and Wiron 99); a Ni-Cr with Cu (CB Soft); a Co-Cr (Wirobond C); and a Cu-base (Thermobond). Alloy specimens (disks 3 x 5 mm) were cast as follows: 100% new alloy; 50% new with 50% recast; and 100% recast (n = 6). Cytotoxicity was tested using Balb/C 3T3 fibroblasts and the MTT assay. Six replicates were tested for each alloy, and the controls consisted of 6 wells containing cells with no alloy specimens. The release of elements from the alloys into culture medium was also investigated. The data were analyzed by 2-way analysis of variance (ANOVA) followed by the Tukey Honestly Significant Difference test (family error rate =.05). RESULTS: At all 3 variations of casting, Remanium CS and Wiron 99 were the least cytotoxic, followed by Wirobond C, CB Soft, and Thermobond. ANOVA revealed significant differences between the groups (P <.001). Post hoc analysis showed that overall, Thermobond was significantly more cytotoxic than all of the other alloys tested (P <.05). For the 100% new alloy groups the cytotoxicity of Co-Cr alloy Wirobond C was similar to that of the Ni-Cralloys Remanium CS and Wiron 99; however, when the alloys were recast, Wirobond C became significantly more cytotoxic and as toxic as the Ni-Cr alloy containing Cu (CB Soft). Moreover, recasting significantly increased the cytotoxicity of all the alloys (P <.05) and the amounts of elements released from them. CONCLUSION: The Cu content in an alloy increased its cytotoxicity level remarkably. Recasting of alloys significantly increased their cytotoxicity level. The Co-Cr alloy was more adversely affected by the recasting than the Ni-Cralloys.