OBJECTIVES: CD36 is a multifunctional membrane receptor widely expressed in different tissues which binds and internalizes oxidized low-density lipoprotein. In rodents, CD36 gene variations modulate glucose homeostasis and contribute to metabolic syndrome associated with type 2 diabetes but the effects in human are unknown. METHODS: We screened the entire coding sequence of the CD36 gene in 272 individuals and we genotyped both rare and frequent variants in 454 T2D subjects and 221 controls. RESULTS: We detected five mutations, P191P and N247S were only found each in one family and did not segregate with diabetes, the three others (A/C-178 in the promoter, A/G-10 in intron 3 and (GGGTTGAGA) insertion in intron 13) being equally frequent in diabetic subjects and in controls. However, adiponectin levels, a marker for insulin sensitivity, were significantly associated with the -178 A/C promoter variant allele (p=0.003, p corrected for multiple testing=0.036), possibly reflecting association with insulin-resistance in the French population. CONCLUSION: Thus, the -178 A/C SNP promoter mutation in the CD36 gene represents a putative genetic marker for insulin-resistance in the French population, although it does not appear to contribute to the genetic risk for T2D.
OBJECTIVES: CD36 is a multifunctional membrane receptor widely expressed in different tissues which binds and internalizes oxidized low-density lipoprotein. In rodents, CD36 gene variations modulate glucose homeostasis and contribute to metabolic syndrome associated with type 2 diabetes but the effects in human are unknown. METHODS: We screened the entire coding sequence of the CD36 gene in 272 individuals and we genotyped both rare and frequent variants in 454 T2D subjects and 221 controls. RESULTS: We detected five mutations, P191P and N247S were only found each in one family and did not segregate with diabetes, the three others (A/C-178 in the promoter, A/G-10 in intron 3 and (GGGTTGAGA) insertion in intron 13) being equally frequent in diabetic subjects and in controls. However, adiponectin levels, a marker for insulin sensitivity, were significantly associated with the -178 A/C promoter variant allele (p=0.003, p corrected for multiple testing=0.036), possibly reflecting association with insulin-resistance in the French population. CONCLUSION: Thus, the -178 A/C SNP promoter mutation in the CD36 gene represents a putative genetic marker for insulin-resistance in the French population, although it does not appear to contribute to the genetic risk for T2D.
Authors: Latisha Love-Gregory; Richard Sherva; Lingwei Sun; Jon Wasson; Timothy Schappe; Alessandro Doria; D C Rao; Steven C Hunt; Samuel Klein; Rosalind J Neuman; M Alan Permutt; Nada A Abumrad Journal: Hum Mol Genet Date: 2008-02-27 Impact factor: 6.150
Authors: Sabrina E Noel; Chao-Qiang Lai; Josiemer Mattei; Laurence D Parnell; Jose M Ordovas; Katherine L Tucker Journal: Atherosclerosis Date: 2010-02-18 Impact factor: 5.162
Authors: Andrew E Fry; Anita Ghansa; Kerrin S Small; Alejandro Palma; Sarah Auburn; Mahamadou Diakite; Angela Green; Susana Campino; Yik Y Teo; Taane G Clark; Anna E Jeffreys; Jonathan Wilson; Muminatou Jallow; Fatou Sisay-Joof; Margaret Pinder; Michael J Griffiths; Norbert Peshu; Thomas N Williams; Charles R Newton; Kevin Marsh; Malcolm E Molyneux; Terrie E Taylor; Kwadwo A Koram; Abraham R Oduro; William O Rogers; Kirk A Rockett; Pardis C Sabeti; Dominic P Kwiatkowski Journal: Hum Mol Genet Date: 2009-04-29 Impact factor: 6.150