Literature DB >> 15671669

Highly efficient mRNA-based gene transfer in feeder-free cultured H9 human embryonic stem cells.

Peter Ponsaerts1, Sjaak van der Sar, Viggo F I Van Tendeloo, Philippe G Jorens, Zwi N Berneman, Prim B Singh.   

Abstract

Several protocols have been described for virus-based gene transfer in human embryonic stem (hES) cells, while efficient non-viral methods are currently non-existing. In this study, we investigated the efficiency of mRNA-based gene transfer in feeder-free cultured H9 hES cells, based on electroporation of in vitro transcribed mRNA encoding the enhanced green fluorescent protein (EGFP). Optimisation of culture and electroporation conditions for feeder-free cultured H9 hES cells resulted a highly pure, transgene-expressing (90% positive cells) H9 hES cell population.

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Year:  2004        PMID： 15671669     DOI： 10.1089/clo.2004.6.211

Source DB:  PubMed          Journal:  Cloning Stem Cells        ISSN： 1536-2302


  6 in total

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Authors:  Feng Cao; Xiaoyan Xie; Timothy Gollan; Li Zhao; Kazim Narsinh; Randall J Lee; Joseph C Wu
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  6 in total

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