Literature DB >> 15668791

Insect-resistant transgenic Pinus radiata.

Lynette J Grace1, Julia A Charity, Belinda Gresham, Nod Kay, Christian Walter.   

Abstract

Transgenic radiata pine (Pinus radiata D. Don) plants containing a Bacillus thuringiensis (Bt) toxin gene, crylAc, were produced by means of biolistic transformation of embryogenic tissue. Using the selectable marker gene nptII and corresponding geneticin selection, 20 independent transgenic lines from five genotypes were established. Over 200 plants regenerated from ten transgenic lines were successfully transferred to soil. The integration and expression of the introduced genes in transgenic tissue and/or plants were confirmed by PCR, Southern hybridisation and neomycin phosphotransferase II (NPTII) and Bt ELISA assays. Bioassays with larvae of the painted apple moth, Teia anartoides, demonstrated that transgenic plants displayed variable levels of resistance to insect damage, with one transgenic line being highly resistant to feeding damage.

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Year:  2005        PMID： 15668791     DOI： 10.1007/s00299-004-0912-x

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  16 in total

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8.  beta-Glucuronidase from Escherichia coli as a gene-fusion marker.

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