OBJECTIVE: To design, implement, and test a selective lung ventilator for setting a rat model of unilateral ventilator-induced lung injury (VILI). DESIGN AND SETTING: Interventional animal study in a university laboratory for animal research. SUBJECTS: Anesthetized and paralyzed male Wistar rats. INTERVENTIONS: A selective ventilator designed to apply varying tidal volume, PEEP, and breathing gas to each lung of the rat was implemented and evaluated. Five control animals were ventilated at 7 ml/kg (3.5 ml/kg each lung). Unilateral VILI was induced in six animals subjected to selective ventilation (3.5 ml/kg in one lung and 15 ml/kg in the other lung). After 3 h of ventilation the animals were killed and the lungs excised. MEASUREMENTS AND RESULTS: Lung edema was assessed by means of the ratio between wet and dry lung weights. No significant differences were found in lungs of control animals (5.16+/-0.22 and 4.96+/-0.25), but the W/D ratio in the over ventilated lung (8.98+/-3.80) was significantly greater than that in the normally ventilated lung (4.76+/-0.15), indicating selective induction of lung edema by over stretch. CONCLUSIONS: This selective ventilator can be implemented into a rat model of unilateral VILI to gain further insight into the mechanisms of pulmonary injury induced by different ventilatory strategies.
OBJECTIVE: To design, implement, and test a selective lung ventilator for setting a rat model of unilateral ventilator-induced lung injury (VILI). DESIGN AND SETTING: Interventional animal study in a university laboratory for animal research. SUBJECTS: Anesthetized and paralyzed male Wistar rats. INTERVENTIONS: A selective ventilator designed to apply varying tidal volume, PEEP, and breathing gas to each lung of the rat was implemented and evaluated. Five control animals were ventilated at 7 ml/kg (3.5 ml/kg each lung). Unilateral VILI was induced in six animals subjected to selective ventilation (3.5 ml/kg in one lung and 15 ml/kg in the other lung). After 3 h of ventilation the animals were killed and the lungs excised. MEASUREMENTS AND RESULTS:Lung edema was assessed by means of the ratio between wet and dry lung weights. No significant differences were found in lungs of control animals (5.16+/-0.22 and 4.96+/-0.25), but the W/D ratio in the over ventilated lung (8.98+/-3.80) was significantly greater than that in the normally ventilated lung (4.76+/-0.15), indicating selective induction of lung edema by over stretch. CONCLUSIONS: This selective ventilator can be implemented into a rat model of unilateral VILI to gain further insight into the mechanisms of pulmonary injury induced by different ventilatory strategies.
Authors: Susana Granell; Anna Serrano-Mollar; Emma Folch-Puy; Daniel Navajas; Ramón Farre; Oriol Bulbena; Daniel Closa Journal: Free Radic Biol Med Date: 2004-11-15 Impact factor: 7.376
Authors: Scott E Sinclair; David A Kregenow; Wayne J E Lamm; Ian R Starr; Emil Y Chi; Michael P Hlastala Journal: Am J Respir Crit Care Med Date: 2002-08-01 Impact factor: 21.405
Authors: Jessica Julioti Urbano; Renata Kelly da Palma; Flávia Mafra de Lima; Paula Fratini; Leticia Lopes Guimaraes; Juan J Uriarte; Letícia Heineck Alvarenga; Maria Angelica Miglino; Rodolfo de Paula Vieira; Renato Araujo Prates; Daniel Navajas; Ramon Farrè; Luis Vicente Franco Oliveira Journal: PLoS One Date: 2017-06-01 Impact factor: 3.240