Literature DB >> 15665484

Purification, characterization, and antifungal activity of chitinases from pineapple (Ananas comosus) leaf.

Toki Taira1, Noriko Toma, Masanobu Ishihara.   

Abstract

Three chitinases, designated pineapple leaf chitinase (PL Chi)-A, -B, and -C were purified from the leaves of pineapple (Ananas comosus) using chitin affinity column chromatography followed by several column chromatographies. PL Chi-A is a class III chitinase having a molecular mass of 25 kDa and an isoelectric point of 4.4. PL Chi-B and -C are class I chitinases having molecular masses of 33 kDa and 39 kDa and isoelectric points of 7.9 and 4.6 respectively. PL Chi-C is a glycoprotein and the others are simple proteins. The optimum pHs of PL Chi-A, -B, and -C toward glycolchitin are pH 3, 4, and 9 respectively. The chitin-binding ability of PL Chi-C is higher than that of PL Chi-B, and PL Chi-A has lower chitin-binding ability than the others. At low ionic strength, PL Chi-B exhibits strong antifungal activity toward Trichoderma viride but the others do not. At high ionic strength, PL Chi-B and -C exhibit strong and weak antifungal activity respectively. PL Chi-A does not have antifungal activity.

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Year:  2005        PMID： 15665484     DOI： 10.1271/bbb.69.189

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  7 in total

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2.  Designing a new chitinase with more chitin binding and antifungal activity.

Authors:  Soheila Matroodi; Mostafa Motallebi; Mohammadreza Zamani; Mehdi Moradyar
Journal:  World J Microbiol Biotechnol       Date:  2013-03-21       Impact factor: 3.312

3.  A class V chitinase from Arabidopsis thaliana: gene responses, enzymatic properties, and crystallographic analysis.

Authors:  Takayuki Ohnuma; Tomoyuki Numata; Takuo Osawa; Mamiko Mizuhara; Outi Lampela; André H Juffer; Karen Skriver; Tamo Fukamizo
Journal:  Planta       Date:  2011-03-09       Impact factor: 4.116

4.  cDNA cloning, expression, and antifungal activity of chitinase from Ficus microcarpa latex: difference in antifungal action of chitinase with and without chitin-binding domain.

Authors:  Tomoya Takashima; Hajime Henna; Dan Kozome; Sakihito Kitajima; Keiko Uechi; Toki Taira
Journal:  Planta       Date:  2021-05-13       Impact factor: 4.116

5.  Gene expression of PLAT and ATS3 proteins increases plant resistance to insects.

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Journal:  Planta       Date:  2021-01-19       Impact factor: 4.116

6.  The pathogenesis-related protein PR-4b from Theobroma cacao presents RNase activity, Ca(2+) and Mg(2+) dependent-DNase activity and antifungal action on Moniliophthora perniciosa.

Authors:  Sara Pereira Menezes; Edson Mario de Andrade Silva; Eline Matos Lima; Aurizângela Oliveira de Sousa; Bruno Silva Andrade; Livia Santos Lima Lemos; Karina Peres Gramacho; Abelmon da Silva Gesteira; Carlos Priminho Pirovani; Fabienne Micheli
Journal:  BMC Plant Biol       Date:  2014-06-11       Impact factor: 4.215

7.  Refolding of β-stranded class I chitinases of Hippophae rhamnoides enhances the antifreeze activity during cold acclimation.

Authors:  Ravi Gupta; Renu Deswal
Journal:  PLoS One       Date:  2014-03-13       Impact factor: 3.240
  7 in total

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