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Literature DB >> 15664445

Imaging protein behavior inside the living cell.

Abstract

The genetically encoded fluorescent proteins (FPs) have transformed studies in cell biology by allowing the behavior of proteins to be tracked within the natural environment of the living cell. Progressively more complex imaging methods are being used to measure the mobility, co-localization and interactions of proteins labeled with the FPs. This review provides an overview of recent developments in live-cell imaging techniques to analyze the subcellular distribution and interactions of proteins in living cells.

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Year: 2005 PMID： 15664445 DOI： 10.1016/j.mce.2004.10.011

Source DB: PubMed Journal: Mol Cell Endocrinol ISSN： 0303-7207 Impact factor: 4.102

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Cited

7 in total

1. Mobility of human immunodeficiency virus type 1 Pr55Gag in living cells.

Authors: Candace Y Gomez; Thomas J Hope
Journal: J Virol Date: 2006-09 Impact factor: 5.103

2. Biosensors of DsRed as FRET partner with CFP or GFP for quantitatively imaging induced activation of Rac, Cdc42 in living cells.

Authors: Rushi Liu; Daoquan Ren; Yizhou Liu; Yuting Deng; Bin Sun; Qingyan Zhang; Xiangrong Guo
Journal: Mol Imaging Biol Date: 2011-06 Impact factor: 3.488

3. Accuracy and precision in quantitative fluorescence microscopy.

Authors: Jennifer C Waters
Journal: J Cell Biol Date: 2009-06-29 Impact factor: 10.539

4. Generation of double-labeled reporter cell lines for studying co-dynamics of endogenous proteins in individual human cells.

Authors: Irina Issaeva; Ariel A Cohen; Eran Eden; Cellina Cohen-Saidon; Tamar Danon; Lydia Cohen; Uri Alon
Journal: PLoS One Date: 2010-10-21 Impact factor: 3.240

Imaging protein behavior inside the living cell.

1. Mobility of human immunodeficiency virus type 1 Pr55Gag in living cells.

2. Biosensors of DsRed as FRET partner with CFP or GFP for quantitatively imaging induced activation of Rac, Cdc42 in living cells.

3. Accuracy and precision in quantitative fluorescence microscopy.

4. Generation of double-labeled reporter cell lines for studying co-dynamics of endogenous proteins in individual human cells.

5. Uptake of aluminium into Arabidopsis root cells measured by fluorescent lifetime imaging.

Review 6. Live cell imaging of the HIV-1 life cycle.

7. Nanostructure Introduces Artifacts in Quantitative Immunofluorescence by Influencing Fluorophore Intensity.