| Literature DB >> 15664068 |
P Saldarelli1, H Keller, M Dell'Orco, A Schots, V Elicio, A Minafra.
Abstract
A panel of 15 recombinant single chain antibodies (scFv) specific to grapevine virus B (GVB) were recovered from a human combinatorial scFv antibody library using the phage display technique against purified virus particles. Two selected scFv-encoding genes were expressed in recombinant Escherichia coli cells as dimeric antibodies. Successful detection of GVB in tissues of herbaceous hosts and grapevine was obtained in a direct binding assay using dimeric scFvs. This reagent was also shown to substitute efficiently for a GVB polyclonal serum in standard DAS-ELISA test used routinely for diagnosis.Entities:
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Year: 2004 PMID： 15664068 DOI： 10.1016/j.jviromet.2004.11.021
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014