Literature DB >> 15662501

Excision of a selectable marker in transgenic rice (Oryza sativa L.) using a chemically regulated Cre/loxP system.

C Sreekala1, L Wu, K Gu, D Wang, D Tian, Z Yin.   

Abstract

Removal of a selectable marker gene from genetically modified (GM) crops alleviates the risk of its release into the environment and hastens the public acceptance of GM crops. Here we report the production of marker-free transgenic rice by using a chemically regulated, Cre/loxP-mediated site-specific DNA recombination in a single transformation. Among 86 independent transgenic lines, ten were found to be marker-free in the T0 generation and an additional 17 lines segregated marker-free transgenic plants in the T1 generation. Molecular and genetic analyses indicated that the DNA recombination and excision in transgenic rice were precise and the marker-free recombinant T-DNA was stable and heritable.

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Year:  2005        PMID： 15662501     DOI： 10.1007/s00299-004-0909-5

Source DB:  PubMed          Journal:  Plant Cell Rep        ISSN： 0721-7714            Impact factor:   4.570


  20 in total

1.  A recombinase-mediated transcriptional induction system in transgenic plants.

Authors:  T Hoff; K M Schnorr; J Mundy
Journal:  Plant Mol Biol       Date:  2001-01       Impact factor: 4.076

2.  Cre recombinase expression can result in phenotypic aberrations in plants.

Authors:  Eric R Coppoolse; Marianne J de Vroomen; Dick Roelofs; Jaap Smit; Femke van Gennip; Bart J M Hersmus; H John J Nijkamp; Mark J J van Haaren
Journal:  Plant Mol Biol       Date:  2003-01       Impact factor: 4.076

3.  Cre recombinase-mediated site-specific recombination between plant chromosomes.

Authors:  M Qin; C Bayley; T Stockton; D W Ow
Journal:  Proc Natl Acad Sci U S A       Date:  1994-03-01       Impact factor: 11.205

4.  A transformation vector for the production of marker-free transgenic plants containing a single copy transgene at high frequency.

Authors:  K Sugita; T Kasahara; E Matsunaga; H Ebinuma
Journal:  Plant J       Date:  2000-06       Impact factor: 6.417

5.  Vectors carrying two separate T-DNAs for co-transformation of higher plants mediated by Agrobacterium tumefaciens and segregation of transformants free from selection markers.

Authors:  T Komari; Y Hiei; Y Saito; N Murai; T Kumashiro
Journal:  Plant J       Date:  1996-07       Impact factor: 6.417

6.  Selectable marker-free transgenic plants without sexual crossing: transient expression of cre recombinase and use of a conditional lethal dominant gene.

Authors:  A P Gleave; D S Mitra; S R Mudge; B A Morris
Journal:  Plant Mol Biol       Date:  1999-05       Impact factor: 4.076

7.  Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum.

Authors:  A M Lloyd; R W Davis
Journal:  Mol Gen Genet       Date:  1994-03

8.  Establishing an efficient Ac/Ds tagging system in rice: large-scale analysis of Ds flanking sequences.

Authors:  Tatiana Kolesnik; Ildiko Szeverenyi; Doris Bachmann; Chellian Santhosh Kumar; Shuye Jiang; Rengasamy Ramamoorthy; Minnie Cai; Zhi Gang Ma; Venkatesan Sundaresan; Srinivasan Ramachandran
Journal:  Plant J       Date:  2004-01       Impact factor: 6.417

9.  Segregation of genes transferred to one plant cell from two separate Agrobacterium strains.

Authors:  T D McKnight; M T Lillis; R B Simpson
Journal:  Plant Mol Biol       Date:  1987-11       Impact factor: 4.076

10.  The Gin recombinase of phage Mu can catalyse site-specific recombination in plant protoplasts.

Authors:  S Maeser; R Kahmann
Journal:  Mol Gen Genet       Date:  1991-11
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  50 in total

1.  Development of a simple and efficient system for excising selectable markers in Arabidopsis using a minimal promoter::Cre fusion construct.

Authors:  Hyun-Bi Kim; Jung-Il Cho; Nayeon Ryoo; Shaohong Qu; Guo-Liang Wang; Jong-Seong Jeon
Journal:  Mol Cells       Date:  2011-11-29       Impact factor: 5.034

Review 2.  Recent advances in development of marker-free transgenic plants: regulation and biosafety concern.

Authors:  Narendra Tuteja; Shiv Verma; Ranjan Kumar Sahoo; Sebastian Raveendar; I N Bheema Lingeshwara Reddy
Journal:  J Biosci       Date:  2012-03       Impact factor: 1.826

3.  Utilization of PVX-Cre expression vector in potato.

Authors:  Lilya Kopertekh; Veronica v Saint Paul; Erika Krebs; Joachim Schiemann
Journal:  Transgenic Res       Date:  2011-09-27       Impact factor: 2.788

4.  Functionality of the beta/six site-specific recombination system in tobacco and Arabidopsis: a novel tool for genetic engineering of plant genomes.

Authors:  Jesper T Grønlund; Christian Stemmer; Jacek Lichota; Thomas Merkle; Klaus D Grasser
Journal:  Plant Mol Biol       Date:  2007-03       Impact factor: 4.076

5.  Evaluation of CRE-mediated excision approaches in Arabidopsis thaliana.

Authors:  Gordana Marjanac; Annelies De Paepe; Ingrid Peck; Anni Jacobs; Sylvie De Buck; Anna Depicker
Journal:  Transgenic Res       Date:  2007-05-31       Impact factor: 2.788

6.  A Cre/loxP-mediated self-activating gene excision system to produce marker gene free transgenic soybean plants.

Authors:  Zhongsen Li; Aiqiu Xing; Bryan P Moon; Susan A Burgoyne; Anthony D Guida; Huiling Liang; Catharina Lee; Cheryl S Caster; Joanne E Barton; Theodore M Klein; Saverio C Falco
Journal:  Plant Mol Biol       Date:  2007-08-22       Impact factor: 4.076

7.  Cre/lox system to develop selectable marker free transgenic tobacco plants conferring resistance against sap sucking homopteran insect.

Authors:  Dipankar Chakraborti; Anindya Sarkar; Hossain A Mondal; David Schuermann; Barbara Hohn; Bidyut K Sarmah; Sampa Das
Journal:  Plant Cell Rep       Date:  2008-07-29       Impact factor: 4.570

8.  Generation of selectable marker-free transgenic tomato resistant to drought, cold and oxidative stress using the Cre/loxP DNA excision system.

Authors:  Yue Zhang; Hua Liu; Bei Li; Jian-Tao Zhang; Yizhou Li; Hongxia Zhang
Journal:  Transgenic Res       Date:  2009-03-05       Impact factor: 2.788

9.  Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system.

Authors:  Hee-Jong Woo; Hyun-Suk Cho; Sun-Hyung Lim; Kong-Sik Shin; Si-Myung Lee; Ki-Jong Lee; Dong-Hern Kim; Yong-Gu Cho
Journal:  Transgenic Res       Date:  2009-01-22       Impact factor: 2.788

10.  Generation of selectable marker-free sheath blight resistant transgenic rice plants by efficient co-transformation of a cointegrate vector T-DNA and a binary vector T-DNA in one Agrobacterium tumefaciens strain.

Authors:  Rajasekaran Sripriya; Vengoji Raghupathy; Karuppannan Veluthambi
Journal:  Plant Cell Rep       Date:  2008-07-29       Impact factor: 4.570
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